Expression of recombinant human lysozyme in transgenic chicken promotes the growth of Bifidobacterium in the intestine and improves postnatal growth of chicken.
Expression of recombinant human lysozyme in transgenic chicken promotes the growth of Bifidobacterium in the intestine and improves postnatal growth of chicken.
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重组人溶菌酶在转基因鸡中表达促进肠道双歧杆菌生长，改善鸡产后生长
DOI：
10.1186/s13568-016-0280-2
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发表时间：
2016-12
期刊：
影响因子：
3.7
通讯作者：
Lian Z
中科院分区：
文献类型：
--
作者：
Wang H;Wu H;Wang K;Cao Z;Yu K;Lian L;Lian Z
溶菌酶是一种抗菌蛋白，常用作饲料添加剂，可防御病原菌，增强动物的免疫功能。本研究将表达重组人溶菌酶（rhLZ）基因的慢病毒载体注射到鸡胚胎胚盘中，探讨重组人溶菌酶对鸡产后肠道菌群分布和生长性能的影响。我们成功培育了 194 只转基因鸡，经 Southern blot 鉴定，转基因阳性率为 24%。蛋清中 rhLZ 的平均浓度为 29.90 ± 6.50 μg/mL。溶菌板测定表明，转基因鸡蛋清中溶菌酶的抗菌活性显着高于非转基因鸡（P < 0.05）。收集转基因和非转基因鸡的粪便，分离培养5种细菌（乳酸菌、沙门氏菌、双歧杆菌、金黄色葡萄球菌和大肠杆菌），检测rhLZ对肠道菌群的影响。在这五种细菌中，转基因者肠道中的双歧杆菌数量显着增加（P < 0.05）。此外，还分析了转基因鸡和非转基因鸡的生长特性。结果发现，转基因鸡6周龄胫长、6周体重、18周体重均较非转基因鸡显着增加。
Lysozyme is one kind of antimicrobial proteins and often used as feed additive which can defend against pathogenic bacteria and enhance immune function of animals. In this study, we have injected the lentiviral vector expressing recombinant human lysozyme (rhLZ) gene into the blastoderm of chicken embryo to investigate the effect of recombinant human lysozyme on postnatal intestinal microbiota distribution and growth performance of chicken. Successfully, we generated 194 transgenic chickens identified by Southern blot with a positive transgenic rate of 24%. The average concentration of rhLZ was 29.90 ± 6.50 μg/mL in the egg white. Lysozyme in egg white of transgenic chickens had a significantly higher antibacterial activity than those of non-transgenic chickens by lysoplate assay (P < 0.05). The feces of transgenic and non-transgenic chickens were collected and five types of bacteria (Lactobacillus, Salmonella, Bifidobacterium, Staphylococcus aureus and Escherichia coli) were isolated and cultured to detect the impact of rhLZ on gut microbiota. Among the five bacteria, the number of Bifidobacterium in the intestine of those transgenic was significantly increased (P < 0.05). Moreover, the growth traits of the transgenic and non-transgenic chickens were analyzed. It was found that the 6-week shank length, 6-week weight and 18-week weight of transgenic chickens were significantly increased than that of non-transgenic chickens.
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影响因子：
3.8
作者：
Byun SJ;Ji MR;Jang YJ;Hwang AI;Chung HK;Kim JS;Kim KW;Chung HJ;Yang BC;Jeon I;Park JK;Yoo JG;Kim TY
通讯作者：
Kim TY
影响因子：
5.4
作者：
Dicks, L. M. T.;Botes, M.
通讯作者：
Botes, M.
影响因子：
3.7
作者：
Kong Q;Wu M;Huan Y;Zhang L;Liu H;Bou G;Luo Y;Mu Y;Liu Z
通讯作者：
Liu Z
DOI：
10.1046/j.1432-1327.1999.00064.x
发表时间：
1999-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Ito, Y;Yoshikawa, A;Imoto, T
通讯作者：
Imoto, T
DOI：
10.1073/pnas.93.13.6659
发表时间：
1996-06-25
影响因子：
11.1
作者：
Dobie, KW;Lee, M;McClenaghan, M
通讯作者：
McClenaghan, M