Post-translational modifications to Toxoplasma gondii alpha- and beta-tubulins include novel C-terminal methylation.
Post-translational modifications to Toxoplasma gondii alpha- and beta-tubulins include novel C-terminal methylation.
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DOI：
10.1021/pr900699a
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发表时间：
2010-01
影响因子：
4.4
通讯作者：
Weiss, Louis M.
中科院分区：
文献类型：
--
作者：
Mao, Hui;El Bissati, Kamal;Verdier-Pinard, Pascal;Burd, Berta;Zhang, Hongshan;Kim, Kami;Fiser, Andras;Angeletti, Ruth Hogue;Weiss, Louis M.
关键词：
刚地弓形虫是一种具有医学和兽医学重要性的顶复合体，被归类为NIH类别B优先病原体。它最为人所知的是它能够在免疫功能正常的宿主中引起先天性感染和在免疫功能低下的宿主中引起脑炎。高度稳定和特化的基于微管的细胞骨架参与入侵过程。该基因组编码α-和β-微管蛋白的三种亚型，并且我们在本文中表明微管蛋白被特异性翻译后修饰（PTM）广泛改变。T.通过质谱和使用特异性抗体的免疫标记分析弓形虫微管蛋白PTM。在α-微管蛋白上鉴定的PTM包括Lys 40的乙酰化、最后一个C-末端氨基酸残基Tyr 453的去除（去酪氨酸微管蛋白）和最后五个氨基酸残基的截短。在α-和β-微管蛋白上均检测到多聚谷氨酰化。一种针对哺乳动物α-微管蛋白（缺少最后两个C-末端残基）的抗体（Δ2-微管蛋白）标记了该寄生虫的顶端区域。脱酪氨酸微管蛋白广泛存在于膜下微管中，并在基底端显示明显的积聚。甲基化，PTM以前没有描述微管蛋白，也被检测到。在宿主细胞，人包皮成纤维细胞中未检测到甲基化微管蛋白，表明这可能是顶复门特有的修饰。
Toxoplasma gondii is an apicomplexan of both medical and veterinary importance which is classified as an NIH Category B priority pathogen. It is best known for its ability to cause congenital infection in immune competent hosts and encephalitis in immune compromised hosts. The highly stable and specialized microtubule-based cytoskeleton participates in the invasion process. The genome encodes three isoforms of both α- and β-tubulin and we show that the tubulin is extensively altered by specific post-translational modifications (PTMs) in this paper. T. gondii tubulin PTMs were analyzed by mass spectrometry and immunolabeling using specific antibodies. The PTMs identified on α-tubulin included acetylation of Lys40, removal of the last C-terminal amino acid residue Tyr453 (detyrosinated tubulin) and truncation of the last five amino acid residues. Polyglutamylation was detected on both α- and β-tubulins. An antibody directed against mammalian α-tubulin lacking the last two C-terminal residues (Δ2-tubulin) labeled the apical region of this parasite. Detyrosinated tubulin was diffusely present in subpellicular microtubules and displayed an apparent accumulation at the basal end. Methylation, a PTM not previously described on tubulin, was also detected. Methylated tubulins were not detected in the host cells, human foreskin fibroblasts, suggesting that this may be a modification specific to the Apicomplexa.
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影响因子：
7.4
作者：
Jung, Sung Yun;Li, Yehua;Qin, Jun
通讯作者：
Qin, Jun
影响因子：
4.4
作者：
Madrid-Aliste CJ;Dybas JM;Angeletti RH;Weiss LM;Kim K;Simon I;Fiser A
通讯作者：
Fiser A
影响因子：
1.5
作者：
NAGEL, SD;BOOTHROYD, JC
通讯作者：
BOOTHROYD, JC
影响因子：
2
作者：
Plessmann, U;Reiter-Owona, I;Lechtreck, KF
通讯作者：
Lechtreck, KF
影响因子：
56.9
作者：
EDDE, B;ROSSIER, J;DENOULET, P
通讯作者：
DENOULET, P