A unified sample preparation protocol for proteomic and genomic profiling of cervical swabs to identify biomarkers for cervical cancer screening.
A unified sample preparation protocol for proteomic and genomic profiling of cervical swabs to identify biomarkers for cervical cancer screening.
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DOI：
10.1002/prca.200780146
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发表时间：
2008-12
影响因子：
2
通讯作者：
Townsend, R. Reid
中科院分区：
文献类型：
--
作者：
Rader, Janet S.;Malone, James P.;Gross, Julia;Gilmore, Petra;Brooks, Rebecca A.;Nguyen, Loan;Crimmins, Dan L.;Feng, Sheng;Wright, Jason D.;Taylor, Nicholas;Zighelboim, Israel;Funk, Margo C.;Huettner, Phyllis C.;Ladenson, Jack H.;Gius, David;Townsend, R. Reid
Cervical cancer screening is ideally suited for the development of biomarkers due to the ease of tissue acquisition and the well-established histological transitions. Furthermore, cell and biologic fluid obtained from cervix samples undergo specific molecular changes that can be profiled. However, the ideal manner and techniques for preparing cervical samples remains to be determined. To address this critical issue a patient screening protein and nucleic acid collection protocol was established. RNAlater was used to collect the samples followed by proteomic methods to identify proteins that were differentially expressed in normal cervical epithelial versus cervical cancer cells. Three hundred ninety spots were identified via two-dimensional difference gel electrophoresis (2-D DIGE) that were expressed at either higher or lower levels (>3-fold) in cervical cancer samples. These proteomic results were compared to genes in a cDNA microarray analysis of microdissected neoplastic cervical specimens to identify overlapping patterns of expression. The most frequent pathways represented by the combined dataset were: cell cycle: G2/M DNA damage checkpoint regulation; aryl hydrocarbon receptor signaling; p53 signaling; cell cycle: G1/S checkpoint regulation; and the endoplasmic reticulum stress pathway. HNRPA2B1 was identified as a biomarker candidate with increased expression in cancer compared to normal cervix and validated by Western blot.
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影响因子：
7.4
作者：
Havlis, J;Thomas, H;Shevchenko, A
通讯作者：
Shevchenko, A
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
254.7
作者：
Parkin, DM;Bray, F;Pisani, P
通讯作者：
Pisani, P
影响因子：
3.4
作者：
Alban, A;David, SO;Currie, I
通讯作者：
Currie, I
影响因子：
2
作者：
McDunn, Jonathan E.;Townsend, R. Reid;Cobb, J. Perren
通讯作者：
Cobb, J. Perren