NXT1 is necessary for the terminal step of Crm1-mediated nuclear export.
NXT1 is necessary for the terminal step of Crm1-mediated nuclear export.
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NXT1对于CRM1介导的核出口的终端步骤是必需的。
DOI：
10.1083/jcb.152.1.141
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发表时间：
2001-01-08
期刊：
影响因子：
--
通讯作者：
Paschal BM
中科院分区：
文献类型：
--
作者：
Black BE;Holaska JM;Lévesque L;Ossareh-Nazari B;Gwizdek C;Dargemont C;Paschal BM
可溶性因子是介导蛋白质和RNA通过核孔复合物（NPC）输出核所必需的。这些可溶性因子包括直接与转运底物结合的受体和决定受体-底物复合物组装状态的调节剂。我们最近报道了NXT 1的鉴定，NXT 1是一种NTF 2相关的输出因子，其刺激透化细胞中的核蛋白输出并在体内经历核质穿梭（Black，B.E.，L. Lévesque，J.M. Holaska，TC Wood和B.M.复活节1999.摩尔Cell. 19：8616-8624）。在这里，我们描述的背景下Crm 1依赖的出口途径的NXT 1的分子特征。我们发现，NXT 1直接结合到Crm 1，并且相互作用是敏感的Ran-GTP的存在。此外，NXT 1中减少与Crm 1结合的突变在核输出测定中抑制NXT 1的活性。我们表明，重组Crm 1和Ran足以重建Rev报告蛋白从核仁到NPC细胞质侧的抗体可及位点的核转位。出口途径的进一步进展，包括Crm 1和Rev报告蛋白释放的最终步骤，需要NXT 1。我们建议NXT 1与核质中的出口复合物接合，并且它促进将出口复合物递送到NPC的细胞质侧上的一个位点，在该位点中受体和底物被释放到细胞质中。
Soluble factors are required to mediate nuclear export of protein and RNA through the nuclear pore complex (NPC). These soluble factors include receptors that bind directly to the transport substrate and regulators that determine the assembly state of receptor–substrate complexes. We recently reported the identification of NXT1, an NTF2-related export factor that stimulates nuclear protein export in permeabilized cells and undergoes nucleocytoplasmic shuttling in vivo (Black, B.E., L. Lévesque, J.M. Holaska, T.C. Wood, and B.M. Paschal. 1999. Mol. Cell. Biol. 19:8616–8624). Here, we describe the molecular characterization of NXT1 in the context of the Crm1-dependent export pathway. We find that NXT1 binds directly to Crm1, and that the interaction is sensitive to the presence of Ran-GTP. Moreover, mutations in NXT1 that reduce binding to Crm1 inhibit the activity of NXT1 in nuclear export assays. We show that recombinant Crm1 and Ran are sufficient to reconstitute nuclear translocation of a Rev reporter protein from the nucleolus to an antibody accessible site on the cytoplasmic side of the NPC. Further progress on the export pathway, including the terminal step of Crm1 and Rev reporter protein release, requires NXT1. We propose that NXT1 engages with the export complex in the nucleoplasm, and that it facilitates delivery of the export complex to a site on the cytoplasmic side of NPC where the receptor and substrate are released into the cytoplasm.
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影响因子：
64.5
作者：
Fornerod, M;Ohno, M;Mattaj, IW
通讯作者：
Mattaj, IW
影响因子：
11.4
作者：
Katahira, J;Strässer, K;Hurt, E
通讯作者：
Hurt, E
影响因子：
5.6
作者：
Clarkson, WD;Corbett, AH;Stewart, M
通讯作者：
Stewart, M
影响因子：
3.4
作者：
Mahajani, SM;Sharma, MM
通讯作者：
Sharma, MM
DOI：
10.1083/jcb.145.2.255
发表时间：
1999-04-19
期刊：
The Journal of cell biology
影响因子：
--
作者：
Paraskeva E;Izaurralde E;Bischoff FR;Huber J;Kutay U;Hartmann E;Lührmann R;Görlich D
通讯作者：
Görlich D