Could humic acid relieve the biochemical toxicities and DNA damage caused by nickel and deltamethrin in earthworms (Eisenia foetida)?
Could humic acid relieve the biochemical toxicities and DNA damage caused by nickel and deltamethrin in earthworms (Eisenia foetida)?
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腐植酸能否缓解镍和溴氰菊酯对蚯蚓（Eisenia foetida）造成的生化毒性和DNA损伤？
DOI：
10.1039/c5em00288e
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发表时间：
2015-12
期刊：
影响因子：
--
通讯作者：
Ming-Yang Wan
中科院分区：
文献类型：
--
作者：
Chen-Chao Shen;Dong-Sheng Shen;Jia-Li Shentu;Mei-Zhen Wang;Ming-Yang Wan
本研究的目的是确定腐殖酸（HA）是否能防止土壤中镍和溴氰菊酯（分别为100和1 mg kg（-1））对蚯蚓的基因和生化毒性效应。通过测量暴露42天以上对脂质膜和DNA的损害以及蛋白质羰基的产生，评价了镍和溴氰菊酯对蚯蚓的细胞和分子水平的毒性效应。镍和溴氰菊酯诱导蚯蚓的氧化应激水平显着，增加生产的过氧化产物（丙二醛和蛋白质羰基），并增加彗星试验尾部DNA%（通过单细胞凝胶电泳测定）。DNA损伤是最敏感的三个指标，因为它提供了一个更高的样品/对照比没有其他指标。HA的存在减轻了镍和溴氰菊酯对DNA、蛋白质和脂膜的损伤（按有效性降序排列）。低剂量HA（土壤中0.5- 1%HA）防止了大量的脂质膜损伤，但最高剂量HA（土壤中3%HA）防止了更多的DNA损伤。然而，蚯蚓中的丙二醛浓度较高，在最高的HA剂量比在较低的HA剂量。在不同HA剂量下产生的蛋白质羰基的量没有显著差异。添加HA可缓解可氧化镍浓度升高对蚯蚓的毒害作用。
The aim of the study was to determine whether humic acid (HA) prevented gene and biochemical toxic effects in earthworms (Eisenia foetida) exposed to nickel and deltamethrin (at 100 and 1 mg kg(-1), respectively) in soil. Cellular- and molecular-level toxic effects of nickel and deltamethrin in earthworms were evaluated by measuring damage to lipid membranes and DNA and the production of protein carbonyls over 42 days of exposure. Nickel and deltamethrin induced significant levels of oxidative stress in earthworms, increasing the production of peroxidation products (malondialdehyde and protein carbonyls) and increasing the comet assay tail DNA% (determined by single-cell gel electrophoresis). DNA damage was the most sensitive of the three indices because it gave a higher sample/control ratio than did the other indices. The presence of HA alleviated (in decreasing order of effectiveness) damage to DNA, proteins, and lipid membranes caused by nickel and deltamethrin. A low HA dose (0.5-1% HA in soil) prevented a great deal of lipid membrane damage, but the highest HA dose (3% HA in soil) prevented still more DNA damage. However, the malondialdehyde concentrations in earthworms were higher at the highest HA dose than at the lower HA doses. The amounts of protein carbonyls produced at different HA doses were not significantly different. The toxic effects to earthworms caused by increased oxidizable nickel concentrations could be relieved by adding HA.
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DOI：
10.1016/s0048-9697(03)00445-5
发表时间：
2004-02
期刊：
The Science of the total environment
影响因子：
--
作者：
Nanke Meems;Christian E. W. Steinberg;Claudia Wiegand
通讯作者：
Nanke Meems;Christian E. W. Steinberg;Claudia Wiegand
影响因子：
2.3
作者：
D. Spurgeon;J. Weeks;C.A.M. van Gestel
通讯作者：
D. Spurgeon;J. Weeks;C.A.M. van Gestel
影响因子：
6.1
作者：
Shengli Wang;Z. Nan;Xiaowen Liu;Yuan Li;Song Qin;Hai-Mai Ding
通讯作者：
Shengli Wang;Z. Nan;Xiaowen Liu;Yuan Li;Song Qin;Hai-Mai Ding
影响因子：
2.8
作者：
S. Lynn;F. Yew;K. Chen;K. Jan
通讯作者：
S. Lynn;F. Yew;K. Chen;K. Jan
蚯蚓（Eisenia fetida）对土壤金霉素污染的生理和分子反应
影响因子：
8.9
作者：
Lin, Dasong;Zhou, Qixing;Li, Ye
通讯作者：
Li, Ye