Developmental phenotypic and transcriptomic effects of exposure to nanomolar levels of metformin in zebrafish.
Developmental phenotypic and transcriptomic effects of exposure to nanomolar levels of metformin in zebrafish.
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斑马鱼暴露于纳摩尔水平的二甲双胍对发育表型和转录组的影响。
DOI：
10.1016/j.etap.2021.103716
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发表时间：
2021-10
影响因子：
4.3
通讯作者：
Baker TR
中科院分区：
文献类型：
--
作者：
Phillips J;Akemann C;Shields JN;Wu CC;Meyer DN;Baker BB;Pitts DK;Baker TR
在美国的大多数湖泊和溪流中都发现了二甲双胍，导致广泛的环境暴露。本研究的结果表明，在关键发育期长期接触二甲双胍会导致斑马鱼(Danio Rerio)存活率下降，斑马鱼是美国国立卫生研究院批准的人类模型。从受精后4小时到受精后5天，持续时间较长的二甲双胍暴露可见明显的异常，但在受精后4-5天短期暴露24小时并未导致任何明显的异常。然而，长时间和短时间的持续时间确实对斑马鱼的运动活动产生了影响，在接触二甲双胍后，与神经和心血管发育有关的几个基因出现了差异表达。在未来的研究中，应进一步研究二甲双胍在行为、基因表达和形态异常方面的变化，以评估它们对人类健康的潜在影响，因为全世界二甲双胍处方的数量不断增加。
Metformin is found in the majority of lakes and streams in the United States, leading to widespread environmental exposure. Results of the present study indicate that extended duration metformin exposure at critical developmental periods leads to decreased survival rates in zebrafish (danio rerio), an NIH approved human model. Significant abnormalities are seen with extended duration metformin exposure from 4 hours post fertilization up to 5 days post fertilization, although short term metformin exposure for 24 hours at 4–5 days post fertilization did not lead to any significant abnormalities. Both extended and short term duration did however have an impact on locomotor activity of zebrafish, and several genes involved in neurological and cardiovascular development were differentially expressed after exposure to metformin. The changes seen in behavior, gene expression and morphological abnormalities caused by metformin exposure should be examined further in future studies in order to assess their potential human health implications as metformin prescriptions continue to increase worldwide.
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评估14个有机溶剂和载体，用于筛查斑马鱼胚胎和幼虫。
影响因子：
3.7
作者：
Maes J;Verlooy L;Buenafe OE;de Witte PA;Esguerra CV;Crawford AD
通讯作者：
Crawford AD
影响因子：
2.6
作者：
Gong L;Goswami S;Giacomini KM;Altman RB;Klein TE
通讯作者：
Klein TE
影响因子：
3.4
作者：
MacPhail, R. C.;Brooks, J.;Padilla, S.
通讯作者：
Padilla, S.
影响因子：
8.9
作者：
Chatterjee, Nivedita;Lee, Hyunho;Choi, Jinhee
通讯作者：
Choi, Jinhee
影响因子：
3.8
作者：
Dach, Katharina;Yaghoobi, Bianca;Lein, Pamela J.
通讯作者：
Lein, Pamela J.