Development and preliminary testing of a translational model of hepatocellular carcinoma for MR imaging and interventional oncologic investigations.
Development and preliminary testing of a translational model of hepatocellular carcinoma for MR imaging and interventional oncologic investigations.
复制标题
DOI：
10.1016/j.jvir.2011.11.018
复制
发表时间：
2012-03
影响因子：
2.9
通讯作者：
Woodrum, David A.
中科院分区：
文献类型：
--
作者：
Thompson, Scott M.;Callstrom, Matthew R.;Knudsen, Bruce;Anderson, Jill L.;Carter, Rickey E.;Grande, Joseph P.;Roberts, Lewis R.;Woodrum, David A.
开发用于磁共振成像和图像引导介入肿瘤学研究的转化性大鼠肝细胞癌（HCC）疾病模型。雄性大鼠接受假手术对照手术 (N=6)、选择性胆管结扎术 (SBDL; N=4) 或胆总管结扎术 (CBDL; N=6)，其中 4 只大鼠接受程序优化，并将 N1S1 细胞注射到 12 只大鼠肝脏的 2-3 个部位。所有大鼠随后均接受 MRI 评估肿瘤的形成和体积。在一组动物中进行肠系膜血管造影和经皮 MR 引导激光消融术（N = 4）。监测动物体重和肝脏测试。收获后，对肝脏进行总体和显微镜分析。在结扎组之间评估肿瘤体积和实验室参数。 MRI 显示对照组、SBDL 组和 CBDL 组中分别有 5/10 (50.0%)、7/8 (87.5%) 和 12/12 (100%) 部位出现高信号 T2 和低信号 T1 病变并诱发肿瘤。各组肿瘤体积差异显着（p<0.02）。肠系膜血管造影显示肿瘤染色增强。临床和实验室评估显示，CBDL 大鼠的体重 (p = 0.01) 和白蛋白 (p<0.01) 显着下降，总胆红素显着增加 (p = 0.02)，但 SBDL 大鼠则不然 (p = 1.0)。组织学检查显示 CBDL 和 SBDL 大鼠在早期纤维化背景下存在具有局部和血管侵犯的高级 HCC。 MR 引导激光消融产生 1-2 厘米的消融区域，其组织学与可逆和不可逆损伤一致。开发了一种生物学相关的大鼠肝细胞癌疾病模型，用于磁共振成像和初步介入肿瘤学应用。
To develop a translational rat hepatocellular carcinoma (HCC) disease model for magnetic resonance imaging and image-guided interventional oncologic investigations. Male rats underwent sham control surgery (N=6), selective bile duct ligation (SBDL; N=4) or common bile duct ligation (CBDL; N=6) with procedure optimization in 4 rats and N1S1 cell injection into 2–3 sites in the liver of 12 rats. All rats subsequently underwent MRI to assess tumor establishment and volume. Mesenteric angiography and percutaneous MR-guided laser ablation of the liver were performed in a subgroup of animals (N=4). Animal weight and liver tests were monitored. After harvesting, the livers were subjected to gross and microscopic analysis. Tumor volume and laboratory parameters were assessed between ligation groups. MRI demonstrated hyperintense T2 and hypointense T1 lesions with tumor induction in 5/10 (50.0%), 7/8 (87.5%) and 12/12 (100%) sites in the control, SBDL and CBDL groups, respectively. Tumor volumes differed significantly by group (p<0.02). Mesenteric angiography demonstrated an enhancing tumor stain. Clinical and laboratory assessment revealed a significant decrease in weight (p = 0.01) and albumin (p<0.01) and increase in total bilirubin (p = 0.02) in CBDL rats but not SBDL rats (p=1.0). Histologic examination showed high-grade HCCs with local and vascular invasion within the context of early fibrosis in CBDL and SBDL rats. MR-guided laser ablation generated a 1–2 cm ablation zone with histology consistent with reversible and irreversible injury. A biologically relevant rat hepatocellular carcinoma disease model was developed for MR imaging and preliminary interventional oncologic applications.
登录
查看更多内容
影响因子：
11.2
作者：
Guo Y;Zhang Y;Klein R;Nijm GM;Sahakian AV;Omary RA;Yang GY;Larson AC
通讯作者：
Larson AC
影响因子：
17.1
作者：
Andersen JB;Factor VM;Marquardt JU;Raggi C;Lee YH;Seo D;Conner EA;Thorgeirsson SS
通讯作者：
Thorgeirsson SS
影响因子：
3.3
作者：
Mertens, Joachim C.;Martin, Ina V.;Geier, Andreas
通讯作者：
Geier, Andreas
DOI：
10.1016/j.jvir.2008.12.408
发表时间：
2009-03-01
影响因子：
2.9
作者：
Ju, Shenghong;McLennan, Gordon;Jayaram, Hiremagalur N.
通讯作者：
Jayaram, Hiremagalur N.
影响因子：
3.8
作者：
Hoffmann, K.;Glimm, H.;Radeleff, B.;Richter, G.;Heining, C.;Schenkel, I.;Zahlten-Hinguranage, A.;Schirrmacher, P.;Schmidt, J.;Buechler, M. W.;Jaeger, D.;von Kalle, C.;Schemmer, P.
通讯作者：
Schemmer, P.