Oroxylin A modulates mitochondrial function and apoptosis in human colon cancer cells by inducing mitochondrial translocation of wild-type p53.
Oroxylin A modulates mitochondrial function and apoptosis in human colon cancer cells by inducing mitochondrial translocation of wild-type p53.
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Oroxylin A 通过诱导野生型 p53 线粒体易位来调节人结肠癌细胞的线粒体功能和细胞凋亡
DOI：
10.18632/oncotarget.7927
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发表时间：
2016-03-29
期刊：
影响因子：
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通讯作者：
Wei L
中科院分区：
文献类型：
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作者：
Qiao C;Lu N;Zhou Y;Ni T;Dai Y;Li Z;Guo Q;Wei L
Oroxylin A是从黄芩根中提取的黄酮类化合物。我们之前已经证明oroxylin A通过线粒体途径诱导人结肠癌细胞凋亡。在本研究中，我们研究了oroxylin a触发的线粒体凋亡途径的潜在机制，p53调节线粒体存活、线粒体DNA完整性和氧化应激保护。我们确定oroxylin A诱导p53线粒体易位并抑制SOD2活性。此外，我们的研究表明，oroxylin A促进HCT-116细胞中p53-Recql4复合物的形成和线粒体易位。最后，我们发现oroxylin A触发细胞质p53激活，从而促进细胞凋亡。在体内也证实了p53的线粒体易位。因此，oroxylin A在人结肠癌细胞中诱导p53的线粒体易位并导致线粒体功能障碍。
Oroxylin A is a flavonoid extracted from the root of Scutellaria baicalensis Georgi. We previously demonstrated that oroxylin A induced apoptosis in human colon cancer cells via the mitochondrial pathway. In the present study, we investigated the underlying mechanisms responsible for the mitochondrial apoptotic pathway triggered by oroxylin A. p53 regulates mitochondrial survival, mitochondrial DNA integrity, and protection from oxidative stress. We determined that oroxylin A induces p53 mitochondrial translocation and inhibits SOD2 activity. Additionally, our studies demonstrate that oroxylin A promotes the formation and mitochondrial translocation of the p53-Recql4 complex in HCT-116 cells. Finally, we showed that oroxylin A triggers cytosolic p53 activation, thereby promoting apoptosis. Mitochondrial translocation of p53 was also validated in vivo. Thus, oroxylin A induces mitochondrial translocation of p53 and leads to mitochondrial dysfunction in human colon cancer cells.
影响因子：
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作者：
Khatun S;Chaube SK;Bhattacharyya CN
通讯作者：
Bhattacharyya CN