Carotenoids play a positive role in the degradation of heterocycles by Sphingobium yanoikuyae.
Carotenoids play a positive role in the degradation of heterocycles by Sphingobium yanoikuyae.
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类胡萝卜素在Sphingobium yanoikuyae 杂环化合物的降解中发挥积极作用
DOI：
10.1371/journal.pone.0039522
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Xu P
中科院分区：
文献类型：
--
作者：
Liu X;Gai Z;Tao F;Tang H;Xu P
微生物氧化降解是去除环境中杂环等污染物的一种潜在途径。在此过程中，不可避免地会产生活性氧或其他氧化剂，并可能对DNA、蛋白质和膜造成损伤，从而降低降解速率。类胡萝卜素可以作为膜整合抗氧化剂，保护细胞免受氧化应激。从卡唑降解细菌Sphingobium yanoikuyae XLDN2-5中克隆并鉴定了类胡萝卜素生物合成途径中涉及的几个基因。另外，菌株XLDN2-5合成的一种黄色色素类胡萝卜素为玉米黄质，由β-胡萝卜素经β-隐黄质合成。在杂环化合物（咔唑<咔唑+苯并噻吩<咔唑+二苯并噻吩）的生物降解过程中，玉米黄质和过氧化氢的产量同时显著增加。这些较高的产量水平与编码植物烯去饱和酶的基因转录增加一致，植物烯去饱和酶是类胡萝卜素生物合成的关键酶之一。黄泡鞘XLDN2-5可以促进类胡萝卜素之一的玉米黄质的合成，玉米黄质可能调节细胞膜流动性和防御细胞内氧化应激。据我们所知，这是首次报道类胡萝卜素在杂环生物降解中的积极作用，同时阐明了鞘蚌属植物类胡萝卜素的生物合成途径。
Microbial oxidative degradation is a potential way of removing pollutants such as heterocycles from the environment. During this process, reactive oxygen species or other oxidants are inevitably produced, and may cause damage to DNA, proteins, and membranes, thereby decreasing the degradation rate. Carotenoids can serve as membrane-integrated antioxidants, protecting cells from oxidative stress. Several genes involved in the carotenoid biosynthetic pathway were cloned and characterized from a carbazole-degrading bacterium Sphingobium yanoikuyae XLDN2-5. In addition, a yellow-pigmented carotenoid synthesized by strain XLDN2-5 was identified as zeaxanthin that was synthesized from β-carotene through β-cryptoxanthin. The amounts of zeaxanthin and hydrogen peroxide produced were significantly and simultaneously enhanced during the biodegradation of heterocycles (carbazole < carbazole + benzothiophene < carbazole + dibenzothiophene). These higher production levels were consistent with the transcriptional increase of the gene encoding phytoene desaturase, one of the key enzymes for carotenoid biosynthesis. Sphingobium yanoikuyae XLDN2-5 can enhance the synthesis of zeaxanthin, one of the carotenoids, which may modulate membrane fluidity and defense against intracellular oxidative stress. To our knowledge, this is the first report on the positive role of carotenoids in the biodegradation of heterocycles, while elucidating the carotenoid biosynthetic pathway in the Sphingobium genus.
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新分离的咔唑降解鞘氨醇单胞菌菌株对二苯并呋喃和二苯并噻吩的共代谢降解
影响因子：
4.4
作者：
Gai, Zhonghui;Yu, Bo;Xu, Ping
通讯作者：
Xu, Ping
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影响因子：
3.2
作者：
Gai, Zhonghui;Wang, Xiaoyu;Xu, Ping
通讯作者：
Xu, Ping
影响因子：
3.2
作者：
Hannibal, L;Lorquin, J;Giraud, E
通讯作者：
Giraud, E
DOI：
10.1016/s0304-4165(97)00083-4
发表时间：
1998-01-08
影响因子：
3
作者：
Deby-Dupont, G;Deby, C;Lamy, M
通讯作者：
Lamy, M
DOI：
10.1080/00021369.1970.10859781
发表时间：
1970-01-01
期刊：
AGRICULTURAL AND BIOLOGICAL CHEMISTRY
影响因子：
--
作者：
KODAMA, K;NAKATANI, S;YAMADA, K
通讯作者：
YAMADA, K