Prevalence of Viral Frequency-Dependent Infection in Coastal Marine Prokaryotes Revealed Using Monthly Time Series Virome Analysis.
Prevalence of Viral Frequency-Dependent Infection in Coastal Marine Prokaryotes Revealed Using Monthly Time Series Virome Analysis.
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DOI：
10.1128/msystems.00931-22
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发表时间：
2023-02-23
期刊：
影响因子：
6.4
通讯作者：
中科院分区：
文献类型：
--
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感染海洋原核生物的病毒对其宿主的多样性和动态有很大的影响。模型系统表明，病毒感染具有频率依赖性，并受到病毒与宿主接触率的限制。然而，目前还不清楚是否频率依赖性感染是普遍存在于丰富的原核种群与不同的时间动态。为了解决这个问题，我们进行了比较，原核和病毒社区使用16 S rRNA扩增和病毒组测序的基础上每月收集的样本2年在日本沿海网站，大坂湾。原核生物和病毒群落动态中观察到的季节性变化表明，病毒的丰度与其预测的宿主门（或类）相关。丰富的原核生物和病毒之间的共现网络分析显示6，423个共现对，表明宿主和病毒丰度的紧密耦合及其“一对多”对应。虽然稳定的优势种，如SAR 11，表现出很少的共存病毒，其病毒的快速继承表明，感染这些人群的病毒不断变化。我们的研究结果表明，频率依赖性的病毒感染盛行于沿海海洋原核生物，无论宿主类群和时间动态。毫无疑问，病毒感染在丰富的海洋原核生物中普遍存在，无论它们的分类群或生长策略如何。然而，在天然原核生物群落中全面评估病毒感染在技术上仍然很困难。在这项研究中，我们研究了丰富的原核生物的病毒感染，通过监测原核生物和病毒群落在富营养化的沿海站点，大坂湾的每月动态。我们比较了社区动态的病毒与他们的推定主机基于基因组的计算机主机预测。我们观察到预测的病毒-宿主对之间的频繁共现，表明病毒感染在丰富的原核生物中普遍存在，无论它们的类群或时间动态如何。这可能表明，丰富的原核生物通过病毒感染的频繁裂解有相当大的贡献，生物地球化学循环和原核生物群落多样性的维持。
Viruses infecting marine prokaryotes have a large impact on the diversity and dynamics of their hosts. Model systems suggest that viral infection is frequency dependent and constrained by the virus-host encounter rate. However, it is unclear whether frequency-dependent infection is pervasive among the abundant prokaryotic populations with different temporal dynamics. To address this question, we performed a comparison of prokaryotic and viral communities using 16S rRNA amplicon and virome sequencing based on samples collected monthly for 2 years at a Japanese coastal site, Osaka Bay. Concurrent seasonal shifts observed in prokaryotic and viral community dynamics indicated that the abundance of viruses correlated with that of their predicted host phyla (or classes). Cooccurrence network analysis between abundant prokaryotes and viruses revealed 6,423 cooccurring pairs, suggesting a tight coupling of host and viral abundances and their “one-to-many” correspondence. Although stable dominant species, such as SAR11, showed few cooccurring viruses, a fast succession of their viruses suggests that viruses infecting these populations changed continuously. Our results suggest that frequency-dependent viral infection prevails in coastal marine prokaryotes regardless of host taxa and temporal dynamics. IMPORTANCE There is little room for doubt that viral infection is prevalent among abundant marine prokaryotes regardless of their taxa or growth strategy. However, comprehensive evaluations of viral infections in natural prokaryotic communities are still technically difficult. In this study, we examined viral infection in abundant prokaryotes by monitoring the monthly dynamics of prokaryotic and viral communities at a eutrophic coastal site, Osaka Bay. We compared the community dynamics of viruses with those of their putative hosts based on genome-based in silico host prediction. We observed frequent cooccurrence among the predicted virus-host pairs, suggesting that viral infection is prevalent in abundant prokaryotes regardless of their taxa or temporal dynamics. This likely indicates that frequent lysis of the abundant prokaryotes via viral infection has a considerable contribution to the biogeochemical cycling and maintenance of prokaryotic community diversity.
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影响因子：
14.9
作者：
Chan PP;Lowe TM
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5.1
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