Magnetic resonance imaging accurately tracks kidney pathology and heterogeneity in the transition from acute kidney injury to chronic kidney disease.
Magnetic resonance imaging accurately tracks kidney pathology and heterogeneity in the transition from acute kidney injury to chronic kidney disease.
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DOI：
10.1016/j.kint.2020.08.021
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发表时间：
2021-01
影响因子：
19.6
通讯作者：
Bennett KM
中科院分区：
文献类型：
--
作者：
Charlton JR;Xu Y;Wu T;deRonde KA;Hughes JL;Dutta S;Oxley GT;Cwiek A;Cathro HP;Charlton NP;Conaway MR;Baldelomar EJ;Parvin N;Bennett KM
急性肾损伤（AKI）增加慢性肾脏疾病（CKD）的风险。然而，很少有工具来检测AKI后的显微组织变化。本研究采用阳离子铁蛋白增强磁共振成像（CFE-MRI）检查AKI向CKD过渡过程中肾脏病理的异质性。成年雄性小鼠服用叶酸后再服用阳离子铁蛋白，并在第4天（AKI）、第4周（CKD-4）或第12周（CKD-12）时安乐死。采用组织学方法和CFE-MRI检查肾脏。在CKD-4和CKD-12组，肾小球数量减少，并观察到小管皮质病变。与对照组相比，AKI、CKD-4和CKD-12组的肾小球表观体积更大。肾小球随年龄增大而肥大。肾小球间距离和肾小球密度与其他MRI指标相结合，以区分AKI和CKD组与对照组。尽管存在显著的异质性，但在区分AKI和CKD与对照组时，非侵入性（基于mri的）指标与侵入性（组织学）指标一样准确。为了评估阳离子铁蛋白对CKD模型的毒性，CKD-4小鼠接受了阳离子铁蛋白治疗，并在一周后进行了检查。除肾、肝、脾铁含量在CKD-4 +阳离子铁蛋白组较高外，不含阳离子铁蛋白的CKD-4组与不含阳离子铁蛋白组相似。因此，我们的研究证明了fe - mri检测全肾病理的准确性和安全性，从而允许开发新的肾脏疾病生物标志物，并为未来在AKI和CKD小鼠模型中的体内纵向研究提供基础，以跟踪肾细胞的命运。
Acute kidney injury (AKI) increases the risk for chronic kidney disease (CKD). However, there are few tools to detect microstructural changes after AKI. Here, cationic ferritin-enhanced magnetic resonance imaging (CFE-MRI) was applied to examine the heterogeneity of kidney pathology in the transition from AKI to CKD. Adult male mice received folic acid followed by cationic ferritin and were euthanized at four days (AKI), four weeks (CKD-4) or 12 weeks (CKD-12). Kidneys were examined by histologic methods and CFE-MRI. In the CKD-4 and CKD-12 groups, glomerular number was reduced and atubular cortical lesions were observed. Apparent glomerular volume was larger in the AKI, CKD-4 and CKD-12 groups compared to controls. Glomerular hypertrophy occurred with ageing. Interglomerular distance and glomerular density were combined with other MRI metrics to distinguish the AKI and CKD groups from controls. Despite significant heterogeneity, the noninvasive (MRI-based) metrics were as accurate as invasive (histological) metrics at distinguishing AKI and CKD from controls. To assess the toxicity of cationic ferritin in a CKD model, CKD-4 mice received cationic ferritin and were examined one week later. The CKD-4 groups with and without cationic ferritin were similar, except the iron content of the kidney, liver, and spleen was greater in the CKD-4 plus cationic ferritin group. Thus, our study demonstrates the accuracy and safety of CFE-MRI to detect whole kidney pathology allowing for the development of novel biomarkers of kidney disease and providing a foundation for future in vivo longitudinal studies in mouse models of AKI and CKD to track nephron fate.
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DOI：
10.1152/ajprenal.00044.2011
发表时间：
2011-06-01
影响因子：
4.2
作者：
Beeman, Scott C.;Zhang, Min;Bennett, Kevin M.
通讯作者：
Bennett, Kevin M.
影响因子：
8
作者：
Chen, Howard H.;Waghorn, Philip A.;Caravan, Peter
通讯作者：
Caravan, Peter
影响因子：
15.9
作者：
DEROUFFIGNAC, C;MOREL, F
通讯作者：
MOREL, F
DOI：
10.1016/0022-5320(72)90087-1
发表时间：
1972-01-01
期刊：
JOURNAL OF ULTRASTRUCTURE RESEARCH
影响因子：
--
作者：
DANON, D;SKUTELSKY, E;GOLDSTEIN, L
通讯作者：
GOLDSTEIN, L
DOI：
10.1056/nejmoa1614329
发表时间：
2017-06-15
期刊：
The New England journal of medicine
影响因子：
--
作者：
Denic A;Mathew J;Lerman LO;Lieske JC;Larson JJ;Alexander MP;Poggio E;Glassock RJ;Rule AD
通讯作者：
Rule AD