The SUN1 splicing variants SUN1_888 and SUN1_916 differentially regulate nucleolar structure
The SUN1 splicing variants SUN1_888 and SUN1_916 differentially regulate nucleolar structure
复制标题
SUN1剪接变体SUN1_888和SUN1_916差异调节核仁结构
作者：
Satomi;E.;Ueda;M.;Katahira;J.;Hieda;M.
核仁结构是高度动态的，并受到严格的调控，以响应内部线索(如代谢率)和外部线索(如施加于细胞的机械力)。尽管核仁的多层结构在很大程度上是由RNA和蛋白质的类液体性质决定的，但调节核仁的形态和数量的机制仍然难以捉摸。核骨架和细胞骨架(LINC)复合体的连接子由内核膜SAD1/UNC-84(SUN)蛋白和外核膜定位蛋白组成。我们以前发现，SUN1蛋白的缺失会影响核仁的形态。本研究重点研究了SUN1剪接变异体在决定核仁形态中的作用。RNA干扰策略表明，主要表达的突变体SUN1_888和SUN1_916对核仁形态至关重要，但在功能上是不同的。此外，SUN1_888或SUN1_916的缺失改变了染色质结构，影响了组蛋白修饰的分布。基于这些结果，我们提出了一个模型，其中LINC复合体通过染色质调节核仁的形态和数量。
The nucleolar structure is highly dynamic and strictly regulated in response to internal cues, such as metabolic rates, and to external cues, such as mechanical forces applied to cells. Although the multilayered nucleolar structure is largely determined by the liquid‐like properties of RNA and proteins, the mechanisms regulating the morphology and number of nucleoli remain elusive. The linker of the nucleoskeleton and cytoskeleton (LINC) complex comprises inner nuclear membrane Sad1/UNC‐84 (SUN) proteins and outer nuclear membrane‐localized nesprins. We previously showed that the depletion of SUN1 proteins affects nucleolar morphologies. This study focuses on the function of SUN1 splicing variants in determining nucleolar morphology. An RNA interference strategy showed that the predominantly expressed variants, SUN1_888 and SUN1_916, were crucial for nucleolar morphology but functionally distinct. In addition, the depletion of either SUN1_888 or SUN1_916 altered the chromatin structure and affected the distribution of histone modifications. Based on these results, we propose a model in which the LINC complex plays a role in modulating nucleolar morphology and numbers via chromatin.
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由于功能亢进，人类心脏中核仁的大小、数量和形态发生变化。
DOI：
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发表时间：
1998
期刊：
Pathology, Research and Practice
影响因子：
--
作者：
M. Neuburger;G. Herget;L. Plaumann;A. Falk;H. Schwalb;C. Adler
通讯作者：
C. Adler
影响因子：
3.3
作者：
van Koningsbruggen S;Gierlinski M;Schofield P;Martin D;Barton GJ;Ariyurek Y;den Dunnen JT;Lamond AI
通讯作者：
Lamond AI
影响因子：
1.2
作者：
Moujaber O;Omran N;Kodiha M;Pié B;Cooper E;Presley JF;Stochaj U
通讯作者：
Stochaj U
影响因子：
3.7
作者：
Rajgor D;Mellad JA;Autore F;Zhang Q;Shanahan CM
通讯作者：
Shanahan CM
影响因子：
14.9
作者：
Hayashi-Takanaka Y;Yamagata K;Wakayama T;Stasevich TJ;Kainuma T;Tsurimoto T;Tachibana M;Shinkai Y;Kurumizaka H;Nozaki N;Kimura H
通讯作者：
Kimura H