Tissue-specific expression of a cloned chick δ-crystallin gene in mouse cells
Tissue-specific expression of a cloned chick δ-crystallin gene in mouse cells
复制标题
克隆鸡 δ-晶状体蛋白基因在小鼠细胞中的组织特异性表达
作者：
H. Kondoh;K. Yasuda;T. S. Okada
晶状体蛋白是脊椎动物晶状体特异性的一组可溶性蛋白质，已被成功地用作研究透镜分化的分子标记。特别是鸡的分化中的透镜细胞中δ-晶体蛋白的合成和积累已被广泛研究（综述见参考文献1-3）。最近，我们克隆了一个连续延伸的鸡δ-晶体蛋白基因，该基因在透镜细胞中特异性表达4。我们已经将这种基因注射到各种小鼠体细胞的细胞核中。δ-晶体蛋白在哺乳动物晶状体中完全缺失，其被γ-晶体蛋白取代，因此这种异种注射系统应允许我们研究组织特异性基因表达。本实验证明：（1）克隆的鸡δ-晶体蛋白基因在小鼠透镜上皮细胞中的表达与在同源鸡细胞中的表达一样有效，（2）小鼠透镜上皮细胞合成的δ-晶体蛋白具有天然分子量，表明该基因转录本正确剪接;（3）δ-晶状体蛋白基因在非晶状体组织中的表达不明显，提示δ-晶状体蛋白基因在异种环境中具有晶状体特异性表达。据我们所知，这是第一次证明克隆的基因在不同物种的同源细胞类型中表现出优先表达。
Crystallins are a group of soluble proteins specific to vertebrate lenses, and have been used successfully as molecular markers for studying lens differentiation. The synthesis and accumulation of δ-crystaIlin in the differentiating lens cells of chick, in particular, have been extensively studied (for review see refs 1–3). Recently, we have cloned a continuous stretch of a chick δ-crystallin gene which is specifically expressed in lens cells4. We have injected this gene into nuclei of various mouse somatic cells. δ-Crystallin is totally absent from mammalian lenses in which it is replaced by γ-crystallin, thus this xenogeneic injection system should allow us to study tissue-specific gene expression. We report here experiments which demonstrate that (1) the cloned δ-crystallin gene of chick is expressed in the mouse lens epithelial cells as efficiently as in the homologous chick cells; (2) δ-crystallin synthesized in the mouse lens epithelium has the native molecular weight, indicating correct splicing of the gene transcripts; (3) the expression is inefficient in the non-lens tissues examined, suggesting lens-specific expression of the δ-crystallin gene in the xenogeneic environment. To our knowledge, this is the first demonstration that a cloned gene shows preferential expression in homologous cell types of different species.
通过 DNA 介导的基因转移改变基因型和表型。
DOI：
10.1126/science.7414320
发表时间：
1980
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Pellicer,A;Robins,D;Wold,B;Sweet,R;Jackson,J;Lowy,I;Roberts,JM;Sim,GK;Silverstein,S;Axel,R
通讯作者：
Axel,R