Augmentation of surgical angiogenesis in vascularized bone allotransplants with host-derived a/v bundle implantation, fibroblast growth factor-2, and vascular endothelial growth factor administration.
Augmentation of surgical angiogenesis in vascularized bone allotransplants with host-derived a/v bundle implantation, fibroblast growth factor-2, and vascular endothelial growth factor administration.
复制标题
DOI：
10.1002/jor.21098
复制
发表时间：
2010-08
影响因子：
2.8
通讯作者：
Bishop, Allen T.
中科院分区：
文献类型：
--
作者：
Larsen, Mikko;Willems, Wouter F.;Pelzer, Michael;Friedrich, Patricia F.;Yaszemski, Michael J.;Bishop, Allen T.
我们以前已经证明，通过在骨内形成一个由骨源性新生血管生成的循环，在没有长期免疫抑制的情况下，活体同种异体骨的实验性移植是可行的。在这项研究中，我们研究的作用，血管生成细胞因子交付与生物可降解微球，以加强这一进程。用显微外科方法将DA大鼠股骨移植到PVG大鼠。将载有缓冲液、碱性成纤维细胞生长因子（FGF）、血管内皮生长因子（VEGF）或两者的聚（D，L-丙交酯-共-乙交酯）微球与血管源性a/v束一起沿着插入髓内。FK-506每日给药14天，然后停药。在第28天，使用氢冲洗测量骨血流量。进行微血管造影、组织学和组织形态计量学分析。FGF+VEGF组的毛细血管密度（35.1%）大于对照组（13.9%）（p<0.05），并且发现从对照、FGF、VEGF到FGF+VEGF的线性趋势（p<0.005）。VEGF（p<0.01）和FGF+VEGF（p<0.05）的骨形成率更高。VEGF或FGF单独增加血流量比组合时更多。所有移植物的组织学排斥反应分级均较低。去除免疫抑制后，局部给予血管和成纤维细胞生长因子可增强血管化骨同种异体移植物中供体来源植入血管的血管生成、骨形成和骨血流。
We have previously shown experimental transplantation of living allogeneic bone to be feasible without long-term immunosuppression by development of a recipient-derived neoangiogenic circulation within bone. In this study we study the role of angiogenic cytokine delivery with biodegradable microspheres to enhance this process. Microsurgical femoral allotransplantation was performed from DA to PVG rats. Poly(D,L-lactide-co-glycolide) microspheres loaded with buffer, basic fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), or both were inserted intramedullarly along with a recipient-derived a/v bundle. FK-506 was administered daily for 14 days, then discontinued. At 28 days, bone blood flow was measured using hydrogen washout. Microangiography, histologic and histomorphometric analysis were performed. Capillary density was greater in the FGF+VEGF group (35.1%) than control (13.9%) (p<0.05), and a linear trend was found from control, FGF, VEGF, to FGF+VEGF (p<0.005). Bone formation rates were greater with VEGF (p<0.01) and FGF+VEGF (p<0.05). VEGF or FGF alone increased blood flow more than when combined. Histology rejection grading was low in all grafts. Local administration of vascular and fibroblast growth factors augments angiogenesis, bone formation and bone blood flow from implanted blood vessels of donor origin in vascularized bone allografts after removal of immunosuppression.
登录
查看更多内容
DOI：
10.1016/0006-291x(92)92277-5
发表时间：
1992-12-15
影响因子：
3.1
作者：
PEPPER, MS;FERRARA, N;MONTESANO, R
通讯作者：
MONTESANO, R
影响因子：
--
作者：
Huang, Zhinong;Nelson, Ehren Robert;Goodman, Stuart B.
通讯作者：
Goodman, Stuart B.
影响因子：
2.8
作者：
Pelzer, Michael;Larsen, Mikko;Bishop, Allen T.
通讯作者：
Bishop, Allen T.
影响因子：
2.8
作者：
Larsen, Mikko;Pelzer, Michael;Bishop, Allen T.
通讯作者：
Bishop, Allen T.
影响因子：
2.8
作者：
Muramatsu, K;Bishop, AT
通讯作者：
Bishop, AT