The proteome of cholesteryl-ester-enriched versus triacylglycerol-enriched lipid droplets.
The proteome of cholesteryl-ester-enriched versus triacylglycerol-enriched lipid droplets.
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DOI：
10.1371/journal.pone.0105047
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Kraemer FB
中科院分区：
文献类型：
--
作者：
Khor VK;Ahrends R;Lin Y;Shen WJ;Adams CM;Roseman AN;Cortez Y;Teruel MN;Azhar S;Kraemer FB
在细胞内，脂质以脂滴（ld）的形式储存，脂滴由中性脂核组成，由磷脂单层和蛋白质外层包围。ld通常会积聚三酰甘油（TAG）和二酰甘油或胆固醇酯（CE），这取决于组织的类型。最近，人们对ld周围的蛋白质越来越感兴趣。已发现的LD蛋白种类繁多，从结构蛋白到代谢酶，参与囊泡运输的蛋白，以及可能在LD形成中起作用的蛋白。以前的蛋白质组学分析主要集中在tag富集的ld上，而ce富集的ld在很大程度上被忽略了。我们的研究比较了甾体源性细胞中ce富集LD和tag富集LD的LD蛋白。在原代大鼠颗粒细胞中，加载HDL以产生富含ce的ld或脂肪酸以产生富含tag的ld，发现61种蛋白质在富含ce的ld中升高，40种蛋白质在富含tag的ld中升高，278种蛋白质的量相似。通过选择性反应监测（SRM）质谱（MS）进一步验证蛋白表达。SRM证实了先前通过串联质量标记ms检测到的27个肽中的25个的表达，SRM证实了几种蛋白质在ce富集的ld中升高，包括中间丝状波形蛋白。这项研究首次比较了ce富集ld上发现的蛋白质与tag富集ld上发现的蛋白质，是更好地了解甾体源性细胞中ce富集ld上发现的蛋白质的第一步。
Within cells, lipids are stored in the form of lipid droplets (LDs), consisting of a neutral lipid core, surrounded by a phospholipid monolayer and an outer layer of protein. LDs typically accumulate either triacylglycerol (TAG) and diacylglycerol or cholesteryl ester (CE), depending on the type of tissue. Recently, there has been an increased interest in the proteins that surround LDs. LD proteins have been found to be quite diverse, from structural proteins to metabolic enzymes, proteins involved in vesicular transport, and proteins that may play a role in LD formation. Previous proteomics analyses have focused on TAG-enriched LDs, whereas CE-enriched LDs have been largely ignored. Our study has compared the LD proteins from CE-enriched LDs to TAG-enriched LDs in steroidogenic cells. In primary rat granulosa cells loaded with either HDL to produce CE-enriched LDs or fatty acids to produce TAG-enriched LDs, 61 proteins were found to be elevated in CE-enriched LDs and 40 proteins elevated in TAG-enriched LDs with 278 proteins in similar amounts. Protein expression was further validated by selected reaction monitoring (SRM) mass spectrometry (MS). SRM verified expression of 25 of 27 peptides that were previously detected by tandem mass tagging MS. Several proteins were confirmed to be elevated in CE-enriched LDs by SRM including the intermediate filament vimentin. This study is the first to compare the proteins found on CE-enriched LDs with TAG-enriched LDs and constitutes the first step in creating a better understanding of the proteins found on CE-enriched LDs in steroidogenic cells.
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影响因子：
9.8
作者：
Beller M;Sztalryd C;Southall N;Bell M;Jäckle H;Auld DS;Oliver B
通讯作者：
Oliver B
影响因子：
6.5
作者：
Kimmel AR;Brasaemle DL;McAndrews-Hill M;Sztalryd C;Londos C
通讯作者：
Londos C
影响因子：
3.4
作者：
Aggarwal, K;Choe, LH;Lee, KH
通讯作者：
Lee, KH
DOI：
10.1074/jbc.m709221200
发表时间：
2008-04-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Bose M;Whittal RM;Miller WL;Bose HS
通讯作者：
Bose HS
影响因子：
64.8
作者：
Guo, Yi;Walther, Tobias C.;Farese, Robert V., Jr.
通讯作者：
Farese, Robert V., Jr.