A rapid point of care immunoswab assay for SARS-CoV detection.
A rapid point of care immunoswab assay for SARS-CoV detection.
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DOI：
10.1016/j.jviromet.2008.05.023
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发表时间：
2008-09
影响因子：
3.1
通讯作者：
Suresh, Mavanur R.
中科院分区：
文献类型：
--
作者：
Kammila, Sriram;Das, Dipankar;Bhatnagar, Pravin K.;Sunwoo, Hoon H.;Zayas-Zamora, Gustavo;King, Malcolm;Suresh, Mavanur R.
严重急性呼吸系统综合征（SARS）的出现导致了世界范围内的几次爆发。早期的诊断测试并不总是确定SARS疑似患者。核衣壳蛋白（NP）是最主要的病毒衍生的结构蛋白，其在感染的第一周期间在血清和鼻咽抽吸物中大量脱落。作为这些努力的一部分，一个简单的，易于使用的免疫芯片方法，通过产生一组单克隆抗体（MAbs），双特异性MAbs和鸡多克隆IgY抗体对SARS冠状病毒核衣壳蛋白（NP）。使用基于MAb的免疫芯片，在盐水和猪鼻咽抽吸物中检测到200 pg/mL的NP浓度，在兔血清中检测到500 pg/mL的NP浓度。基于BsMAb的免疫拭子检测到NP浓度在盐水中为20 pg/mL，在兔血清中为500 pg/mL，在猪鼻咽抽吸物中为20-200 pg/mL。基于多克隆IgY的免疫拭子在猪鼻咽抽吸物中检测到10 pg/mL的NP浓度，提供了最灵敏的SARS现场护理测定。结果表明，免疫芯片检测SARS-CoV NP抗原的方法可以发展成为一种简单有效的方法，在未来的SARS流行中识别SARS疑似个体，从而减少和遏制传播。这种简单的免疫芯片诊断检测的关键特征是它能够在45-60分钟内检测到SARS-CoV抗原的存在，并提供体液样本。
The emergence of severe acute respiratory syndrome (SARS) resulted in several outbreaks worldwide. Early tests for diagnosis were not always conclusive in identifying a SARS suspected patient. Nucleocapsid protein (NP) is the most predominant virus derived structural protein which is shed in high amounts in serum and nasopharyngeal aspirate during the first week of infection. As part of such efforts, a simple, easy to use immunoswab method was developed by generating a panel of monoclonal antibodies (MAbs), Bispecific MAbs and chicken polyclonal IgY antibody against the SARS-CoV nucleocapsid protein (NP). Employing the MAb-based immunoswab, an NP concentration of 200 pg/mL in saline and pig nasopharyngeal aspirate, and 500 pg/mL in rabbit serum were detected. BsMAb-based immunoswabs detected an NP concentration of 20 pg/mL in saline, 500 pg/mL in rabbit serum and 20–200 pg/mL in pig nasopharyngeal aspirate. Polyclonal IgY-based immunoswabs detected an NP concentration of 10 pg/mL in pig nasopharyngeal aspirate providing the most sensitive SARS point of care assay. Results show that the robust immunoswab method of detecting SARS-CoV NP antigen can be developed into an easy and effective way of identifying SARS suspected individuals during a future SARS epidemic, thereby reducing and containing the transmission. The key feature of this simple immunoswab diagnostic assay is its ability to detect the presence of the SARS-CoV antigen within 45–60 min with the availability of the body fluid samples.
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Vero E6细胞中SARS-核纳病毒的生长动力学。
DOI：
10.1016/j.bbrc.2005.02.085
发表时间：
2005-04-15
影响因子：
3.1
作者：
Keyaerts E;Vijgen L;Maes P;Neyts J;Van Ranst M
通讯作者：
Van Ranst M
影响因子：
5
作者：
HISCOX, JA;CAVANAGH, D;BRITTON, P
通讯作者：
BRITTON, P
影响因子：
3.1
作者：
Das D;Suresh MR
通讯作者：
Suresh MR
DOI：
10.1128/cdli.12.1.135-140.2005
发表时间：
2005-01-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Di, B;Hao, W;Che, XY
通讯作者：
Che, XY
影响因子：
9.4
作者：
Che, XY;Qiu, LW;Yuen, KY
通讯作者：
Yuen, KY