Structural basis for activity of highly efficient RNA mimics of green fluorescent protein.
Structural basis for activity of highly efficient RNA mimics of green fluorescent protein.
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DOI：
10.1038/nsmb.2865
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发表时间：
2014-08
影响因子：
16.8
通讯作者：
Ferré-D'Amaré AR
中科院分区：
文献类型：
--
作者：
Warner KD;Chen MC;Song W;Strack RL;Thorn A;Jaffrey SR;Ferré-D'Amaré AR
Green fluorescent protein (GFP) and its derivatives revolutionized the study of proteins. Spinach is a recently reported in vitro evolved RNA mimic of GFP, which as genetically encoded fusions, makes possible live-cell, real-time imaging of biological RNAs, without resorting to large RNA-binding protein-GFP fusions. To elucidate the molecular basis of Spinach fluorescence, we have solved its co-crystal structure bound to its cognate exogenous chromophore, revealing that Spinach activates the small molecule by immobilizing it between a base triple, a G-quadruplex, and an unpaired guanine. Mutational and NMR analyses indicate that the G-quadruplex is essential for Spinach fluorescence, is also present in other fluorogenic RNAs, and may represent a general strategy for RNAs to induce fluorescence of chromophores. The structure has guided the design of a miniaturized 'Baby Spinach', and provides the foundation for structure-driven design and tuning of fluorescent RNAs.
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DOI：
10.1126/science.1207339
发表时间：
2011-07-29
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Paige JS;Wu KY;Jaffrey SR
通讯作者：
Jaffrey SR
影响因子：
3.7
作者：
Arpino JA;Rizkallah PJ;Jones DD
通讯作者：
Jones DD
影响因子：
15
作者：
Babendure, JR;Adams, SR;Tsien, RY
通讯作者：
Tsien, RY
RNA 双链体-四链体连接的 FMRP RGG 肽识别的结构功能研究。
影响因子：
16.8
作者：
Anh Tuan Phan;Kuryavyi, Vitaly;Darnell, Jennifer C.;Serganov, Alexander;Majumdar, Ananya;Ilin, Serge;Raslin, Tanya;Polonskaia, Anna;Chen, Cynthia;Clain, David;Darnell, Robert B.;Patel, Dinshaw J.
通讯作者：
Patel, Dinshaw J.
DOI：
10.1126/science.1218298
发表时间：
2012-03-09
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Paige JS;Nguyen-Duc T;Song W;Jaffrey SR
通讯作者：
Jaffrey SR