Evolution of foot-and-mouth disease virus intra-sample sequence diversity during serial transmission in bovine hosts.
Evolution of foot-and-mouth disease virus intra-sample sequence diversity during serial transmission in bovine hosts.
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DOI：
10.1186/1297-9716-44-12
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发表时间：
2013-03-01
影响因子：
4.4
通讯作者：
Haydon DT
中科院分区：
文献类型：
--
作者：
Morelli MJ;Wright CF;Knowles NJ;Juleff N;Paton DJ;King DP;Haydon DT
样本中的RNA病毒群体具有高度异质性，含有大量少数序列变异体，这些变异体可能会传播给其他易感宿主。因此，共有基因组序列提供了一个不完整的图片内和宿主之间的病毒传播过程中的进化动力学。口蹄疫病毒（Foot-and-mouth disease virus，FMDV）是一种RNA病毒，其可以从主要复制位点通过体循环传播到上皮表面的不同局部感染位点。这些不同组织中的病毒进化独立发生，它们中的每一个都可能提供病毒来源以播种随后的传播事件。本研究采用Illumina基因组分析仪平台对从一系列连续感染牛中收集的18份FMDV样品进行测序。这些数据生成了不同动物和组织中病毒种群结构演变的快照。对突变谱的分析显示，这些样品在基因组中的21至146个位点处的频率>0.5%的多态性，而13个位点获得超过共有频率（50%）的突变。多态性频率的分析表明，一些少数变异体在宿主间感染事件中传播，而宿主内创始人群体的大小似乎较小。这些数据表明，病毒群体的复杂性受到小的宿主内瓶颈和相对大的宿主间瓶颈的影响。少数变异体的动态与遗传漂变而不是强选择的作用是一致的。这些结果提供了新的见解FMDV的演变，可应用于重建内和宿主间的传播途径。
RNA virus populations within samples are highly heterogeneous, containing a large number of minority sequence variants which can potentially be transmitted to other susceptible hosts. Consequently, consensus genome sequences provide an incomplete picture of the within- and between-host viral evolutionary dynamics during transmission. Foot-and-mouth disease virus (FMDV) is an RNA virus that can spread from primary sites of replication, via the systemic circulation, to found distinct sites of local infection at epithelial surfaces. Viral evolution in these different tissues occurs independently, each of them potentially providing a source of virus to seed subsequent transmission events. This study employed the Illumina Genome Analyzer platform to sequence 18 FMDV samples collected from a chain of sequentially infected cattle. These data generated snap-shots of the evolving viral population structures within different animals and tissues. Analyses of the mutation spectra revealed polymorphisms at frequencies >0.5% at between 21 and 146 sites across the genome for these samples, while 13 sites acquired mutations in excess of consensus frequency (50%). Analysis of polymorphism frequency revealed that a number of minority variants were transmitted during host-to-host infection events, while the size of the intra-host founder populations appeared to be smaller. These data indicate that viral population complexity is influenced by small intra-host bottlenecks and relatively large inter-host bottlenecks. The dynamics of minority variants are consistent with the actions of genetic drift rather than strong selection. These results provide novel insights into the evolution of FMDV that can be applied to reconstruct both intra- and inter-host transmission routes.
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影响因子：
4.9
作者：
Clement, M;Posada, D;Crandall, KA
通讯作者：
Crandall, KA
DOI：
10.2460/javma.2002.220.1636
发表时间：
2002-06-01
期刊：
JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION
影响因子：
--
作者：
Callahan, JD;Brown, F;Nelson, WM
通讯作者：
Nelson, WM
影响因子：
--
作者：
EIGEN, M;SCHUSTER, P
通讯作者：
SCHUSTER, P
影响因子：
3.1
作者：
Kampmann, Marie-Louise;Fordyce, Sarah L.;Gilbert, M. Thomas P.
通讯作者：
Gilbert, M. Thomas P.
影响因子：
14.9
作者：
Hoffmann C;Minkah N;Leipzig J;Wang G;Arens MQ;Tebas P;Bushman FD
通讯作者：
Bushman FD