House dust mite exposure enhances immune responses to ovalbumin-induced intestinal allergy.
House dust mite exposure enhances immune responses to ovalbumin-induced intestinal allergy.
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DOI：
10.1038/s41598-022-09196-8
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发表时间：
2022-03-25
影响因子：
4.6
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Lin J;Chen D;Guan L;Chang K;Li D;Sun B;Yang P;Liu Z
屋尘螨（House dust mites， HDM）是气道变应性疾病的重要因素之一，在人体肠道黏膜中可检出HDM变应原，引起局部炎症，增加肠上皮通透性。本研究验证了一种假说，即HDM与卵清蛋白（OVA）诱导的肠道过敏有关。采用UniCAP100检测食物过敏患者血清抗HDM IgE水平（瑞典乌普萨拉Pharmacia）；以OVA和HDM为特异性抗原建立食物过敏小鼠模型。与健康对照相比，食物过敏患者血清中hdm特异性IgE水平较高。与单独食物过敏组相比，食物过敏和哮喘或变应性鼻炎患者的hdm特异性IgE水平明显更高。在小鼠模型中，我们发现HDM/OVA诱导过敏样症状，降低体温和体重。HDM和HDM + CT（霍乱毒素）组IgE、IgG1、mMCP-1（小鼠肥大细胞蛋白酶-1）、IL-4、IL-5水平均高于对照组，HDM、OVA和HDM + OVA组IgE、IgG1、IL-4、IL-5水平均高于对照组。HDM和HDM + CT组/ HDM、OVA和HDM + OVA组肠道组织病理改变较对照组更为严重，嗜酸性粒细胞浸润较多。此外，暴露于HDM诱导肠道屏障功能障碍，促进肠道过敏的发生。总之，HDM暴露增强了对ova诱导的食物过敏的免疫反应。
House dust mites (HDM) are one of the important factors of airway allergic diseases, HDM allergens can be detected in the human gut mucosa, which induces local inflammation and increases intestinal epithelial permeability. This study tests a hypothesis that HDM contribute to the development of OVA (ovalbumin)-induced intestinal allergy. The serum levels of IgE against HDM in patients with food allergy were detected with UniCAP100 (Pharmacia, Uppsala, Sweden); a mouse model of food allergy was developed with OVA and HDM as the specific antigens. Compared to healthy controls, patients with food allergy have higher levels of serum HDM-specific IgE. Compared to food allergy alone groups, the levels of HDM-specific IgE in patients with food allergy and asthma or allergic rhinitis were significantly higher. In mouse models, we found that HDM/OVA induced allergy-like symptoms, lower body temperature, and lower body weight. The levels of IgE, IgG1, mMCP-1 (mouse mast cell protease-1), IL-4 and IL-5 in the HDM and HDM + CT (cholera toxin) groups were higher than the control groups, and the levels of IgE, IgG1, IL-4 and IL-5 in the HDM, OVA and HDM + OVA groups were higher than the control groups. The pathological changes of intestinal tissues in the HDM and HDM + CT/the HDM, OVA and HDM + OVA groups were more severe, more eosinophil infiltration than the control groups. Moreover, exposure to HDM induced intestinal barrier dysfunction, and facilitated the development of intestinal allergy in mice. In conclusion, HDM exposure enhances immune responses to OVA-induced food allergy.
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DOI：
10.1073/pnas.0813036106
发表时间：
2009-03-03
影响因子：
11.1
作者：
Brandl, Katharina;Rutschmann, Sophie;Beutler, Bruce
通讯作者：
Beutler, Bruce
影响因子：
24.5
作者：
Tulic, Meri K.;Vivinus-Nebot, Mylene;Verhasselt, Valerie
通讯作者：
Verhasselt, Valerie
影响因子：
3.6
作者：
Yang, Yang;Chen, Zhong-Wei;Liu, Guang-Ming
通讯作者：
Liu, Guang-Ming
影响因子：
82.9
作者：
Kim D;Kim YG;Seo SU;Kim DJ;Kamada N;Prescott D;Chamaillard M;Philpott DJ;Rosenstiel P;Inohara N;Núñez G
通讯作者：
Núñez G
影响因子：
4.6
作者：
Li LJ;Zeng L;Li XX;Mo LH;Geng XR;Zheng PY;Liu ZG;Feng BS;Yang PC
通讯作者：
Yang PC