Induction of colitis in mice with food allergen-specific immune response.
Induction of colitis in mice with food allergen-specific immune response.
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DOI：
10.1038/srep32765
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发表时间：
2016-09-08
影响因子：
4.6
通讯作者：
Yang PC
中科院分区：
文献类型：
--
作者：
Li LJ;Zeng L;Li XX;Mo LH;Geng XR;Zheng PY;Liu ZG;Feng BS;Yang PC
肠道慢性炎症的发病机制尚不清楚。食物过敏在肠道炎症的诱发中起重要作用。本研究旨在验证食物过敏引发结肠炎的假设。在这项研究中，BALB/c小鼠致敏一种常见的食物过敏原，卵清蛋白（OVA）与霍乱毒素（CT）作为佐剂。采用Ussing小室技术评价结肠上皮屏障功能。流式细胞术、RT-PCR和Western blotting检测树突状细胞中T细胞免疫球蛋白粘蛋白结构域分子4（TIM 4）的表达。结果显示，小鼠结肠黏膜炎性细胞大量浸润，体重减轻，结肠组织中髓过氧化物酶、肿瘤坏死因子-α、白细胞介素-4、卵清蛋白特异性IgE水平升高。结肠炎组小鼠结肠粘膜屏障功能明显受损，这是通过将结肠粘膜暴露于Ussing室中来模拟的。结肠炎小鼠结肠粘膜中检测到高频率的TIM 4+树突状细胞。树突状细胞暴露于CT显着增加TIM 4的表达。我们的结论是IBD样炎症可以诱导小鼠结肠食物过敏原相关的免疫反应。
The pathogenesis of intestinal chronic inflammation is unclear. Food allergy plays an important role in the induction of intestinal inflammation. This study aims to test a hypothesis that food allergy initiates colitis. In this study, BALB/c mice were sensitized to a common food allergen, ovalbumin (OVA) with cholera toxin (CT) as an adjuvant. The colon epithelial barrier function was assessed with Ussing chamber technique. Expression of T cell immunoglobulin mucin domain molecule-4 (TIM4) in dendritic cells was evaluated by flow cytometry, RT-PCR and Western blotting. The results showed that allergen-related colitis was induced in mice as shown by heavy infiltration of inflammatory cells in the colon mucosa, loss of body weight of mice, increases in myeloperoxidase, tumor necrosis factor-α, interleukin-4, OVA-specific IgE in the colon tissue. The colon epithelial barrier function was markedly compromised in colitis group mice, which was mimicked by exposure the colon mucosa to CT in Ussing chamber. High frequency of TIM4+ dendritic cells was detected in the colon mucosa of colitis mice. Exposure of dendritic cells to CT markedly increased the expression of TIM4. We conclude that IBD-like inflammation can be induced in the mouse colon by the food allergen-related immune response.
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