Effects of three different PAHs on nitrogen-fixing bacterial diversity in mangrove sediment
Effects of three different PAHs on nitrogen-fixing bacterial diversity in mangrove sediment
复制标题
三种不同PAHs对红树林沉积物固氮细菌多样性的影响
DOI：
10.1007/s10646-012-0946-8
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发表时间：
2012-06
期刊：
影响因子：
2.7
通讯作者：
Deng C.
中科院分区：
文献类型：
--
作者：
Wang Y.S.;Sun C.C.;Peng Y.L.;Deng C.
多环芳烃（PAHs）由于其广泛存在、持久性和致癌性，已成为环境和人类健康的重要问题。现在有令人信服的证据表明，红树林沉积物的微生物结构容易受到多环芳烃污染。研究了多环芳烃对红树林沉积物中固氮细菌群落的影响。三种类型的多环芳烃，萘（NAP），二环多环芳烃;芴（FLU），三环多环芳烃;和芘（PYR），四环多环芳烃;应用在三个剂量。培养7天和24天后，用thenifH基因聚合酶链反应变性梯度凝胶电泳图谱证明了固氮细菌的种群和多样性。DGGE图谱显示，固氮细菌群落结构随多环芳烃的种类、浓度和培养时间的变化而发生显著变化。就单一多环芳烃而言，高浓度的多环芳烃对固氮菌的影响比低浓度的多环芳烃更大。此外，在三种多环芳烃中，NAP的短期毒性最大，PYR的长期毒性最强，而FLU的长期毒性相对较高。多维标度分析和对应分析是研究固氮菌群落与多环芳烃污染关系的两种可靠的多元分析方法。研究多环芳烃对红树林沉积物中固氮细菌多样性的影响，有助于了解红树林沉积物中氮的生态地球化学循环过程。固氮细菌群落可以作为表征多环芳烃对红树林沉积物生态系统影响的一个重要参数。
Polycyclic aromatic hydrocarbons (PAHs) are of great environmental and human health concerns due to their widespread occurrence, persistence and carcinogenic properties. There is now compelling evidence that the mangrove sediment microbial structure is susceptible to PAHs contamination. The study aimed to assess the effects of PAHs on the nitrogen-fixing bacterial community of mangrove sediment. Three types of PAHs, naphthalene (NAP), a two-ring PAH; fluorene (FLU), a three-ring PAH; and pyrene (PYR), a four-ring PAH; were applied at three doses. After 7 and 24 days of incubation, the nitrogen-fixing bacterial population and diversity were evidenced in thenifH gene polymerase chain reaction denaturing gradient gel electrophoresis profile. DGGE pattern shows that the nitrogen-fixing bacterial community changed significantly with the types and doses of PAHs, and the incubation time. As far as single PAH is concerned, high concentration of PAH has larger impact on the nitrogen-fixing bacteria than low concentration of PAH. Besides, among the three types of PAHs, NAP has the greatest short term toxicity; PYR has the strongest long-term impact, whereas FLU has relatively higher long-time effect. Multidimensional scaling analysis and correspondence analysis are two reliable multivariate analysis methods for investigating the relationship between the nitrogen-fixing bacterial community and PAHs contamination. Investigating the effect of PAHs on the nitrogen-fixing bacterial diversity could yield useful information for understanding the process of biogeochemical cycling of nitrogen in mangrove sediment. The present study reveals that nitrogen-fixing bacterial community can be used as an important parameter indicating the impact of PAHs on mangrove sediment ecosystem.
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影响因子：
4.4
作者：
S. Churchill;J. P. Harper;P. Churchill
通讯作者：
S. Churchill;J. P. Harper;P. Churchill
影响因子：
4.4
作者：
HICKS, BJ;SILVESTER, WB
通讯作者：
SILVESTER, WB
影响因子：
4.8
作者：
van Bruggen, AHC;Semenov, AM
通讯作者：
Semenov, AM
影响因子：
4.4
作者：
Zhou, JZ;Bruns, MA;Tiedje, JM
通讯作者：
Tiedje, JM
DOI：
10.1007/978-94-011-5636-3_7
发表时间：
1997
期刊：
--
影响因子：
--
作者：
R. H. Burris;G. P. Roberts
通讯作者：
R. H. Burris;G. P. Roberts