Ultrastructural demonstration and analytical application of extrajunctional receptors of denervated human and rat skeletal muscle fibres
Ultrastructural demonstration and analytical application of extrajunctional receptors of denervated human and rat skeletal muscle fibres
复制标题
去神经人类和大鼠骨骼肌纤维的结外受体的超微结构演示和分析应用
作者：
S. Ringel;A. Bender;B. Festoff;W. Engel;Z. Vogel;M. Daniels
我们描述了一种基于肌膜中乙酰胆碱 (ACh) 受体分布差异的可视化区分去神经骨骼肌纤维和正常骨骼肌纤维的新方法。 ACh1,2 的离子电渗应用和标记的 α-银环蛇毒素 (αBuTX)3,4 放射自显影显示，去神经支配后（和神经支配前 5）ACh 受体出现在整个肌膜上，这与它们在神经肌肉接头中的正常位置形成鲜明对比。使用免疫过氧化酶染色技术 6,7 来定位与 ACh 受体 3,4,8 特异性结合的 αBuTX，我们现在拥有一种快速方便的方法，可以在光和电子显微镜水平上高分辨率地观察存在于有缺陷的神经支配的人和大鼠骨骼肌纤维的整个肌膜膜上的 ACh 受体。我们使用这种免疫过氧化物酶分子探测方法来分析各种人类神经肌肉疾病和动物模型的肌肉活检。
WE describe a new approach for distinguishing denervated from normal skeletal muscle fibres, based on the visualisation of differences in distribution of acetylcholine (ACh) receptors in the sarcolemma. Iontophoretic application of ACh1,2 and autoradiography with labelled α-bungarotoxin (αBuTX)3,4 have shown that after denervation (and before innervation5) ACh receptors appear throughout the sarcolemmal membrane, in contrast to their normal location in the neuromuscular junction. Using an immunopeiroxidase staining technique6,7 to localise αBuTX, which binds specifically to ACh receptors3,4,8, we now have a rapid and convenient method for high resolution, at light and electron microscopic levels, of the ACh receptors present throughout the sarcolemmal membrane of defectively innervated human and rat skeletal muscle fibres. We have used this immunoperoxidase method of molecular probing to analyse muscle biopsies in various human neuromuscular diseases and animal models.
影响因子：
3.2
作者：
GRAHAM, RC;KARNOVSKY, MJ
通讯作者：
KARNOVSKY, MJ
影响因子：
3.2
作者：
MCLEAN, IW;NAKANE, PK
通讯作者：
NAKANE, PK