Development of rapid immunochromatographic test for hemagglutinin antigen of H7 subtype in patients infected with novel avian influenza A (H7N9) virus.
Development of rapid immunochromatographic test for hemagglutinin antigen of H7 subtype in patients infected with novel avian influenza A (H7N9) virus.
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DOI：
10.1371/journal.pone.0092306
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Tang S
中科院分区：
文献类型：
--
作者：
Kang K;Chen L;Zhao X;Qin C;Zhan Z;Wang J;Li W;Dzakah EE;Huang W;Shu Y;Jiang T;Cao W;Xie M;Luo X;Tang S
自2013年3月中国发现人感染新型H7N9禽流感病毒以来，相对较高的死亡率和人传人的可能性突显了诊断H7N9感染的敏感和特异检测的迫切需要。在免疫层析检测系统中，我们利用针对H7的抗血凝素(HA)单抗建立了一种新型禽流感病毒(H7N9)的快速诊断试验。H7N9病毒的检出限为103.5 pfu/ml或103TCID50。该方法特异性地检测到H7N9病毒分离株和包括H7N7和H7N9在内的H7亚型的重组HA蛋白，但不与H1N1、H3N1、H5N1、H5N9和H9N2等非H7亚型发生反应。RT-PCR检测H7N9病毒的灵敏度为59.4%(19/32)。H7N9阳性痰标本的敏感性最高，为61.5%(16/26)，鼻咽拭子阳性率为35.7%(5/14)，粪便标本阳性率为20%(2/10)。对180份H7N9阴性标本进行检测，未发现假阳性。我们新的快速检测方法可以特异性地检测H7HA抗原，便于快速诊断以预防和控制正在进行的H7N9疫情。
Since human infection with the novel H7N9 avian influenza virus was identified in China in March 2013, the relatively high mortality rate and possibility of human-to-human transmission have highlighted the urgent need for sensitive and specific assays for diagnosis of H7N9 infection. We developed a rapid diagnostic test for the novel avian influenza A (H7N9) virus using anti-hemagglutinin (HA) monoclonal antibodies specifically targeting H7 in an immunochromatographic assay system. The assay limit of detection was 103.5 pfu/ml or 103TCID50 of H7N9 virus. The assay specifically detected H7N9 viral isolates and recombinant HA proteins of H7 subtypes including H7N7 and H7N9, but did not react with non-H7 subtypes including H1N1, H3N2, H5N1, H5N9, and H9N2. The detection sensitivity was 59.4% (19/32) for H7N9 patients confirmed by RT-PCR. Moreover, the highest sensitivity of 61.5% (16/26) was obtained when testing H7N9 positive sputum samples while 35.7% (5/14) of nasopharyngeal swabs and 20% (2/10) of fecal samples tested positive. No false positive detection was found when testing 180 H7N9 negative samples. Our novel rapid assay can specifically detect H7 HA antigen, facilitating rapid diagnosis for prevention and control of the on-going H7N9 epidemic.
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DOI：
10.1136/bmj.f4752
发表时间：
2013-08-06
期刊：
BMJ (Clinical research ed.)
影响因子：
--
作者：
Qi X;Qian YH;Bao CJ;Guo XL;Cui LB;Tang FY;Ji H;Huang Y;Cai PQ;Lu B;Xu K;Shi C;Zhu FC;Zhou MH;Wang H
通讯作者：
Wang H
人类感染新型禽源甲型流感 (H7N9) 病毒。
影响因子：
158.5
作者：
Gao, Rongbao;Cao, Bin;Shu, Yuelong
通讯作者：
Shu, Yuelong
过去、现在和未来可能的人类感染甲型流感病毒 H7 亚型。
影响因子：
11.8
作者：
Belser, Jessica A.;Bridges, Carolyn B.;Katz, Jacqueline M.;Tumpey, Terrence M.
通讯作者：
Tumpey, Terrence M.
影响因子：
11.8
作者：
Hirst M;Astell CR;Griffith M;Coughlin SM;Moksa M;Zeng T;Smailus DE;Holt RA;Jones S;Marra MA;Petric M;Krajden M;Lawrence D;Mak A;Chow R;Skowronski DM;Tweed SA;Goh S;Brunham RC;Robinson J;Bowes V;Sojonky K;Byrne SK;Li Y;Kobasa D;Booth T;Paetzel M
通讯作者：
Paetzel M
DOI：
10.1186/2043-9113-2-5
发表时间：
2012-02-13
期刊：
Journal of clinical bioinformatics
影响因子：
--
作者：
Wulff NH;Tzatzaris M;Young PJ
通讯作者：
Young PJ