Clarifying Prehistoric Parasitism from a Complementary Morphological and Molecular Approach.
Clarifying Prehistoric Parasitism from a Complementary Morphological and Molecular Approach.
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DOI：
10.1016/j.jas.2013.03.010
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发表时间：
2013-07-01
影响因子：
2.8
通讯作者：
Lewis CM Jr
中科院分区：
文献类型：
--
作者：
Cleeland LM;Reichard MV;Tito RY;Reinhard KJ;Lewis CM Jr
This paper reports an approach to the identification of prehistoric parasitic infection, which integrates traditional morphological methods with molecular methods. The approach includes the strengths of each method while mitigating the limitations. Demonstrating the efficacy of this approach, we provide a case study from a 1,400 year old desiccated fecal sample from La Cueva de los Muertos Chiquitos, archaeological site, near Rio Zape, Durango, Mexico. Traditionally prepared microscope slides were processed via microscopy and tentative ascarids were identified. Information regarding the parasites’ developmental stage was recorded. DNA was then extracted directly from the slide material. From this DNA extract, a small segment of the 18S ribosomal RNA gene variant that is specific to Ascaris, and its phylogenetically close relatives, was targeted for PCR amplification and sequencing. Phylogenetic analysis of the DNA sequence best matched a member of physalopterids, rather than ascarids, with a single exception of a match to Contracaecum spiculigerum. Subsequent extractions, amplifications and sequencing of the original rehydrated coprolite material confirmed these results. The C. spiculigerum sequence represented a phylogenetic anomaly and subsequent analysis determined the sequence was an error in the BLAST database, likely attributable to misidentification of juvenile specimens prior to sequencing and submission. Physaloptera are a difficult genus to identify morphologically and can carry major health burdens. They may be underreported in humans, in part, because of morphological similarities to the more common human parasites belonging to ascarids. We conclude that integrating traditional morphological methods with molecular methods can help resolve this issue, in both contemporary and prehistoric populations.
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影响因子：
4
作者：
Iniguez, Alena Mayo;Reinhard, Karl;Vicente, Ana Carolina Paulo
通讯作者：
Vicente, Ana Carolina Paulo
影响因子：
2.8
作者：
BROOKS, RH;KAPLAN, L;WHITAKER, TW
通讯作者：
WHITAKER, TW
DOI：
10.1016/j.cvex.2009.07.002
发表时间：
2009-09-01
期刊：
The veterinary clinics of North America. Exotic animal practice
影响因子：
--
作者：
Johnson-Delaney, Cathy A
通讯作者：
Johnson-Delaney, Cathy A
DOI：
10.1590/s0074-02762003000900013
发表时间：
2003-01-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Iñiguez, Alena M;Reinhard, Karl J;Vicente, Ana Carolina P
通讯作者：
Vicente, Ana Carolina P
DOI：
10.1590/s0074-02762003000900012
发表时间：
2003-01-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Iñiguez, Alena M;Araújo, Adauto;Vicente, Ana Carolina P
通讯作者：
Vicente, Ana Carolina P