Effects of trichostatins on differentiation of murine erythroleukemia cells.
Effects of trichostatins on differentiation of murine erythroleukemia cells.
复制标题
曲古抑素对小鼠红白血病细胞分化的影响。
作者：
M. Yoshida;S. Nomura;T. Beppu
从钝顶链霉菌No.145的培养液中分离出抑真菌抗生素阿伐他汀（TS）A和C，并发现它们是小鼠红白血病（Friend和RV 133）细胞的有效分化诱导剂，TSA的浓度为1.5 × 10（-8）M，TSC的浓度为5 × 10（-7）M。TS诱导的分化协同紫外线照射，但不与二甲基亚砜处理增强。这种增强的活性被完全抑制，加入放线菌酮暴露于TS后2小时的培养基中，这表明TS是二甲基亚砜型诱导红系分化。TS对培养细胞大分子合成无抑制作用。
The fungistatic antibiotics trichostatins (TS) A and C were isolated from culture broth of Streptomyces platensis No. 145 and were found to be potent inducers of differentiation in murine erythroleukemia (Friend and RV133) cells at concentrations of 1.5 X 10(-8) M for TSA and 5 X 10(-7) M for TSC. Differentiation induced by TS was cooperatively enhanced by UV irradiation but not by treatment with dimethyl sulfoxide. This enhanced activity was completely inhibited by adding cycloheximide to the culture medium 2 h after exposure to TS, suggesting that TS are dimethyl sulfoxide-type inducers of erythroid differentiation. No inhibitory effect of TS was observed on macromolecular synthesis in cultured cells.
通过凝集素结合和凝集检测小鼠红白血病细胞分化过程中表面结构的变化。
DOI：
10.1016/0005-2736(81)90014-6
发表时间：
1981
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Sartorelli,AC
通讯作者：
Sartorelli,AC