Overexpression of α1,6-fucosyltransferase in hepatoma enhances expression of Golgi phosphoprotein 2 in a fucosylation-independent manner.
Overexpression of α1,6-fucosyltransferase in hepatoma enhances expression of Golgi phosphoprotein 2 in a fucosylation-independent manner.
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DOI：
10.3892/ijo.2011.1005
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发表时间：
2011-07
影响因子：
5.2
通讯作者：
Miyoshi E
中科院分区：
文献类型：
--
作者：
Kawamoto S;Moriwaki K;Nakagawa T;Terao M;Shinzaki S;Yamane-Ohnuki N;Satoh M;Mehta AS;Block TM;Miyoshi E
高尔基体磷蛋白2（GP 73）是一种II型高尔基体蛋白，是在检测岩藻糖基化蛋白质组时发现的，是肝细胞癌（HCC）的潜在肿瘤标志物。HCC患者血清中总GP 73和岩藻糖基化GP 73的水平均升高。岩藻糖基化是癌症中最重要的寡糖修饰之一，由α 1，6-岩藻糖基转移酶（Fut 8）催化。在本研究中，我们研究了Fut 8过表达对人肝癌细胞系Hep 3B中GP 73产生的影响。将Fut 8真核表达载体转染Hep 3B细胞，Western blotting和real-time PCR检测GP 73的表达。Fut 8的过表达显著增强了GP 73在转录水平上的表达。令人惊讶的是，这种作用不依赖于细胞岩藻糖基化。过表达的突变体Fut 8，这是无法本地化的高尔基体，不诱导GP 73的生产，这表明在高尔基体的Fut 8的本地化是重要的GP 73的表达增加。这是第一次证明GP 73通过糖基转移酶的过度表达进行调节，这可能导致高尔基体应激。
Golgi phosphoprotein 2 (GP73) is a type II Golgi protein, which was found on examination of the fucosylated proteome as a potential tumor marker for hepatocellular carcinoma (HCC). The serum levels of both total and fucosylated GP73 were increased in the sera of patients with HCC. Fucosylation is one of the most important oligosaccharide modifications involved in cancer and is catalyzed by α1,6-fucosyltransferase (Fut8). In the present study, we investigated the effect of Fut8 overexpression on GP73 production in the human hepatoma cell line Hep3B. The Fut8 expression vector was transfected into Hep3B cells and the expression of GP73 was investigated by Western blotting and real-time PCR. Overexpression of Fut8 dramatically enhanced the expression of GP73 at the transcriptional level. Surprisingly, this effect was not dependent on cellular fucosylation. Overexpression of a mutant Fut8, which was unable to be localized to the Golgi, did not induce GP73 production, suggesting that the localization of Fut8 in the Golgi apparatus was important for the increase in GP73 expression. This is the first demonstration of GP73 regulation through overexpression of a glycosyltransferase, which may lead to Golgi stress.
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影响因子：
13.5
作者：
Riener, Marc-Oliver;Stenner, Frank;Kristiansen, Glen
通讯作者：
Kristiansen, Glen
影响因子：
6.4
作者：
Okuyama, N;Ide, Y;Miyoshi, E
通讯作者：
Miyoshi, E
影响因子：
4
作者：
Norton, Pamela A.;Comunale, Mary Ann;Block, Timothy M.
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Block, Timothy M.
影响因子：
13.5
作者：
Noda, K;Miyoshi, E;Taniguchi, N
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Taniguchi, N
影响因子：
3.5
作者：
Kladney RD;Bulla GA;Guo L;Mason AL;Tollefson AE;Simon DJ;Koutoubi Z;Fimmel CJ
通讯作者：
Fimmel CJ