GP73, a novel Golgi-localized protein upregulated by viral infection.
GP73, a novel Golgi-localized protein upregulated by viral infection.
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DOI：
10.1016/s0378-1119(00)00136-0
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发表时间：
2000-05-16
期刊：
影响因子：
3.5
通讯作者：
Fimmel CJ
中科院分区：
文献类型：
--
作者：
Kladney RD;Bulla GA;Guo L;Mason AL;Tollefson AE;Simon DJ;Koutoubi Z;Fimmel CJ
我们报道了一种新的73 kDa人高尔基蛋白GP73的分离和鉴定。GP73 cDNA是通过对来自成人巨细胞肝炎（GCH）患者肝脏的cDNA文库的差异筛选克隆出来的，GCH是一种罕见的肝炎，推测是病毒病因。体外转录-翻译研究表明GP73是一个完整的膜蛋白，使用表位标记的GP73免疫定位实验表明该蛋白定位于高尔基体。对来自多个人体组织的RNA进行Northern blot分析，发现一个大小约为3.0 kb的GP73 mRNA转录物。利用兔多克隆抗血清对重组GP73进行的免疫组化研究表明，该蛋白在许多人体组织的上皮细胞中优先表达。在正常肝脏中，GP73始终存在于胆道上皮细胞中，而肝细胞很少或没有信号。相比之下，GCH患者的肝脏在多核肝细胞中显示出较强的GP73免疫反应性。体外研究了腺病毒感染HepG2高分化肝癌细胞后GP73 mRNA和蛋白的表达。我们得出结论，GP73代表了一种新的上皮细胞特异性整体膜高尔基蛋白，可以在病毒感染反应中上调。
We report the isolation and characterization of GP73, a novel 73 kDa human Golgi protein. The GP73 cDNA was cloned by differential screening of a cDNA library derived from the liver of a patient with adult giant-cell hepatitis (GCH), a rare form of hepatitis with presumed viral etiology. In vitro transcription–translation studies indicate that GP73 is an integral membrane protein, and immunolocalization experiments using epitope-tagged GP73 demonstrate that the protein is localized to the Golgi apparatus. Northern blot analysis of RNA from multiple human tissues reveals a single GP73 mRNA transcript with a size of approximately 3.0 kb. Immunohistochemical studies using rabbit polyclonal antisera directed against recombinant GP73 demonstrate that the protein is preferentially expressed by epithelial cells in many human tissues. In normal livers, GP73 is consistently present in biliary epithelial cells, whereas hepatocytes show little or no signal. In contrast, livers of patients with GCH display strong GP73 immunoreactivity in multinucleated hepatocytes. GP73 mRNA and protein are expressed in highly differentiated HepG2 hepatoma cells after infection with adenovirus in vitro. We conclude that GP73 represents a novel, epithelial cell-specific integral membrane Golgi protein that can be upregulated in response to viral infection.
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DOI：
10.1073/pnas.90.24.12045
发表时间：
1993-12-15
影响因子：
11.1
作者：
DAIGLE, I;LI, C
通讯作者：
LI, C
影响因子：
64.5
作者：
CARLIN, CR;TOLLEFSON, AE;WOLD, WSM
通讯作者：
WOLD, WSM
影响因子：
9.8
作者：
Fimmel, CJ;Guo, LS;Mason, AL
通讯作者：
Mason, AL
DOI：
10.1083/jcb.124.4.619
发表时间：
1994-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Frisch SM;Francis H
通讯作者：
Francis H
影响因子：
5.4
作者：
Fish, KN;Britt, W;Nelson, JA
通讯作者：
Nelson, JA