CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid Vertebrates.
CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid Vertebrates.
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CRISPR/Cas9 在多倍体脊椎动物基因复制命运调查中的应用
DOI：
10.3389/fgene.2018.00260
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发表时间：
2018
影响因子：
3.7
通讯作者：
Luo J
中科院分区：
文献类型：
--
作者：
Yin F;Liu W;Chai J;Lu B;Murphy RW;Luo J
多倍化广泛存在于真核生物中，尤其是在植物中。多倍体植物和一些鱼类已经商业化。通常，在多倍化之后会立即发生严重的基因组扰动，对于多倍体后代如何在遗传和表观遗传学变化中存活下来，人们知之甚少。对此的研究需要鉴定与多倍化相关的基因，并区分父本和母本的剂量平衡，而不考虑同源多倍化或异源多倍化的机制。新的途径和技术可能会识别通过异源多倍化中父本和母本基因的重组而获得的新基因功能的镶嵌。用CRISPR相关系统蛋白9(CRISPR/Cas9)修饰簇状规则间隔短回文重复序列(CRISPR/Cas9)已被用于植物多倍化的研究。然而，这种方法很少被应用于脊椎动物的多倍化。在这里，我们使用CRISPR/Cas9来追踪四倍体金鱼的基因命运，特别是鉴定fgf20a的两个不同拷贝的功能分化，这两个拷贝在整个胚胎发育过程中表达不同。我们期待该基因编辑系统能够应用于多倍体研究和多倍体家畜遗传改良。
Polyploidization occurs widely in eukaryotes, and especially in plants. Polyploid plants and some fishes have been commercialized. Typically, severe genomic perturbations immediately follow polyploidization and little is known about how polyploid offspring survives the genetic and epigenetic changes. Investigations into this require the identification of genes related to polyploidization and the discrimination of dosage-balance from paternal and maternal copies, and regardless of the mechanism being either autopolyploidization or allopolyploidization. New approaches and technologies may discern the mosaic of novel gene functions gained through the recombination of paternal and maternal genes in allopolyploidization. Modifications of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) with CRISPR-associated system (Cas) protein 9 (CRISPR/Cas9) have been employed in studies of polyploidization of plants. However, the approach has seldom been applied to polyploidization in vertebrates. Herein, we use CRISPR/Cas9 to trace gene-fate in tetraploid goldfish, and specifically to identify the functional differentiation of two divergent copies of fgf20a, which are expressed differently throughout embryonic development. We expect this gene editing system will be applicable to studies of polyploids and the genetic improvement of polyploid livestock.
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影响因子：
5.3
作者：
Kim B;Yu HJ;Park SG;Shin JY;Oh M;Kim N;Mun JH
通讯作者：
Mun JH
影响因子：
64.8
作者：
Matsumoto, T;Wu, JZ;Sugiura, M
通讯作者：
Sugiura, M
金鱼与鲤鱼杂交的二倍体和四倍体后代的基因组不相容性
DOI：
10.1073/pnas.1512955113
发表时间：
2016-02-02
影响因子：
11.1
作者：
Liu, Shaojun;Luo, Jing;Zhang, Ya-Ping
通讯作者：
Zhang, Ya-Ping
DOI：
10.1016/0027-5107(90)90024-x
发表时间：
1990-08-01
期刊：
MUTATION RESEARCH
影响因子：
--
作者：
MAYER, VW;AGUILERA, A
通讯作者：
AGUILERA, A
影响因子：
16.6
作者：
Liao, Hsin-Kai;Gu, Ying;Belmonte, Juan Carlos Izpisua
通讯作者：
Belmonte, Juan Carlos Izpisua