Role of growth hormone and insulin-like growth factor-I in mammary development.
Role of growth hormone and insulin-like growth factor-I in mammary development.
复制标题
生长激素和胰岛素样生长因子-I 在乳腺发育中的作用。
作者：
Karen Plaut;M. Ikeda;B. Vonderhaar
Mammary glands from 3- to 4-week-old mice were incubated in whole organ culture to determine the effects of GH, PRL, and insulin-like growth factor-I (IGF-I) on lobulo-alveolar development and milk protein expression. Virgin mice were implanted with pellets of estrogen and progesterone (1:1000). After 9 days, abdominal no. 4 glands were removed and place on siliconized lens paper in Waymouths' medium supplemented with insulin (Ins), aldosterone, hydrocortisone, and epidermal growth factor. Concentrations of bovine GH, ovine GH, rat GH, or ovine PRL added to the medium varied from 0-1 micrograms/ml. IGF-I was added to replace either Ins or PRL up to 1 microgram/ml. When glands were incubated with Ins, aldosterone, hydrocortisone, and 250 ng/ml PRL, they exhibited lobulo-alveolar development and expressed the milk protein beta-casein. When GH was substituted for PRL, little lobulo-alveolar development occurred, although beta-casein mRNA was expressed at low levels. Either PRL or GH at 1 microgram/ml induced lobulo-alveolar development and beta-casein mRNA. Addition of epidermal growth factor to whole organ culture with GH or PRL (1 microgram/ml) was equally effective in stimulating lobulo-alveolar development. IGF-I did not substitute for PRL, GH, or insulin in tissue maintenance. It is clear that GH at high concentrations can act directly on mouse mammary tissue to induce both lobulo-alveolar development and casein expression.
泌乳素依赖性胰岛素样生长因子结合蛋白在培养的小鼠乳腺上皮细胞中的表达。
影响因子：
4.8
作者：
Fielder,PJ;Thordarson,G;English,A;Rosenfeld,RG;Talamantes,F
通讯作者：
Talamantes,F
牛生长激素的结晶和初步 X 射线表征。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Bell,JA;Moffat,K;Vonderhaar,BK;Golde,DW
通讯作者：
Golde,DW
催乳激素增加小鼠乳腺表皮生长因子信使 RNA 的含量。
DOI：
10.1016/0006-291x(91)92045-l
发表时间：
1991
影响因子：
3.1
作者：
Fenton,SE;Sheffield,LG
通讯作者：
Sheffield,LG