Deoxyribonucleic Acid Relatedness Among Selected Strains of Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium bovis BCG, Mycobacterium microti, and Mycobacterium africanum
Deoxyribonucleic Acid Relatedness Among Selected Strains of Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium bovis BCG, Mycobacterium microti, and Mycobacterium africanum
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结核分枝杆菌、牛分枝杆菌、牛分枝杆菌 BCG、田鼠分枝杆菌和非洲分枝杆菌选定菌株之间的脱氧核糖核酸相关性
DOI：
10.1099/00207713-35-2-147
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发表时间：
1985
影响因子：
2.8
通讯作者：
T. Imaeda
中科院分区：
文献类型：
--
作者：
T. Imaeda
选择的结核分枝杆菌复合体菌株的脱氧核糖核酸（DNA），包括M.结核分枝杆菌、牛分枝杆菌、M.牛BCG、田鼠分枝杆菌和非洲分枝杆菌进行分离和荧光定量分析。DNA样品的基因组分子量和鸟嘌呤+胞嘧啶含量分别约为1.9 × 109和62.9mol%。DNA分光杂交和限制性内切酶酶切方法研究菌株之间的DNA亲缘关系。除了M. microti和M. bovis、牛黑脉菌M. bovis BCG或M.非洲的。用限制性酶（例如EcoRI、EcoRV、BglII、BclI和KpnI）产生的DNA片段的分布模式在琼脂糖凝胶电泳上无法区分。相反，M.结核分枝杆菌和其他生长缓慢的分枝杆菌，包括非产色分枝杆菌、胞内分枝杆菌、堪萨斯分枝杆菌、鸟分枝杆菌、结核分枝杆菌、嗜血分枝杆菌、海分枝杆菌和亚洲分枝杆菌，具有显著的异质性，范围为9 - 53%。我的数据表明，四种M之间的进化关系非常密切。肺结核综合征
Deoxyribonucleic acids (DNAs) of selected strains of the Mycobacterium tuberculosis complex, including strains of M. tuberculosis, Mycobacterium bovis, M. bovis BCG, Mycobacterium microti, and Mycobacterium africanum, were isolated and analyzed spectrophotometrically. The genome molecular weight and the guanine-plus-cytosine content of the DNA samples were approximately 1.9 × 109and 62.9 mol%, respectively. DNA relatedness among the strains was studied by both spectrophometric DNA hybridization and restriction endonuclease cleavage methods. All strains of the four species showed more than 90% DNA relatedness, except for slightly lower levels (85 to 89%) of relatedness between M. microti and some strains of M. bovis, M. bovis BCG, or M. africanum. The distribution patterns of the DNA fragments produced with restriction enzymes, such as EcoRI, EcoRV, BglII, BclI, and KpnI, were indistinguishable upon agarose gel electrophoresis. In contrast, the levels of DNA relatedness between M. tuberculosis and other slowly growing mycobacteria, including Mycobacterium nonchromogenicum, Mycobacterium intracellulare, Mycobacterium kansasii, Mycobacterium avium, Mycobacterium scrofulaceum, Mycobacterium haemophilum, Mycobacterium marinum, and Mycobacterium asiaticum, were significantly heterogeneous, ranging from 9 to 53%. My data indicate the extremely close evolutionary relationship among four species of the M. tuberculosis complex.
根据限制性内切酶数据估计 DNA 水平的遗传变异。
DOI：
10.1073/pnas.78.6.3748
发表时间：
1981
影响因子：
11.1
作者：
Ewens,WJ;Spielman,RS;Harris,H
通讯作者：
Harris,H