Adherence to and penetration through endothelial cells by oral treponemes.
Adherence to and penetration through endothelial cells by oral treponemes.
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口腔密螺旋体粘附并渗透内皮细胞。
DOI：
10.1034/j.1399-302x.1999.140609.x
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发表时间：
1999
影响因子：
--
通讯作者：
D. D. Thomas
中科院分区：
文献类型：
--
作者：
S. Peters;M. Valdez;G. Riviere;D. D. Thomas
将口腔螺旋体与接种到多孔板或安装在塑料室中的过滤器上的单层内皮细胞共培养。使用酶联免疫吸附测定和扫描电子显微镜评估附着。通过使用暗场显微镜监测室内过滤器下方的介质中的螺旋体来确定侵袭性。使用透射电子显微镜来估计螺旋体通过单层的细胞间和细胞内通道。除噬菌体密螺旋体外，所有测试的密螺旋体均以剂量和时间依赖性方式附着在单层上。在宿主细胞的细胞质内观察到一些密螺旋体。从牙菌斑中获得的不明螺旋体也具有侵入性。结果表明，口腔螺旋体具有与致病螺旋体共有的毒力相关特征。进一步的研究应该检查侵入性螺旋体从受影响的牙龈内传播的可能性。
Oral spirochetes were co-incubated with monolayers of endothelial cells seeded into multiwell plates or onto filters mounted in plastic chambers. Attachment was assessed using an enzyme-linked immunosorbent assay and scanning electron microscopy. Invasiveness was determined by monitoring media beneath filters within chambers for spirochetes using darkfield microscopy. Transmission electron microscopy was used to estimate intercellular and intracellular passage of spirochetes through monolayers. All tested treponemes attached to monolayers in a dose- and time-dependent manner, except Treponema phagedenis. A few treponemes were observed within host cell cytoplasm. Unidentified spirochetes obtained from dental plaque were also invasive. Results indicate that oral spirochetes possess virulence-associated characteristics shared with pathogenic spirochetes. Further studies should examine the possibility that invasive spirochetes could disseminate from within affected gingiva.
DOI：
10.1073/pnas.85.10.3608
发表时间：
1988-05-01
影响因子：
11.1
作者：
THOMAS, DD;NAVAB, M;LOVETT, MA
通讯作者：
LOVETT, MA