Bioconjugated nanoparticle detection of respiratory syncytial virus infection.
Bioconjugated nanoparticle detection of respiratory syncytial virus infection.
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DOI：
10.2147/nano.2007.2.1.117
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发表时间：
2007
影响因子：
8
通讯作者：
Chen W
中科院分区：
文献类型：
--
作者：
Tripp RA;Alvarez R;Anderson B;Jones L;Weeks C;Chen W
纳米技术与生物学的结合在分子诊断学、治疗学和生物工程方面取得了重大进展。最近的进展导致功能化纳米颗粒（NPs）的发展，它们与生物分子如抗体、肽、蛋白质和核酸共价连接。这些功能化的NPs有助于开发新的诊断工具和方法，特别是针对病原体的诊断工具和方法，因为快速和敏感的诊断对于确定感染的出现、确定应采取预防措施的时间、评价药物和疫苗的功效以及控制流行病至关重要。在本研究中，我们证明了与单克隆抗体结合的功能化NPs可用于体外和体内快速特异性检测呼吸道合胞病毒。这些结果表明，功能化的NPs可以提供直接、快速、灵敏的病毒检测，从而弥补了目前繁琐的病毒检测方法与对更快速、灵敏的病毒试剂检测的迫切需求之间的差距。
The integration of nanotechnology with biology has produced major advances in molecular diagnostics, therapeutics, and bioengineering. Recent advances have led to the development of functionalized nanoparticles (NPs) that are covalently linked to biological molecules such as antibodies, peptides, proteins, and nucleic acids. These functionalized NPs allow for development of novel diagnostic tools and methods, particularly for pathogens, as rapid and sensitive diagnostics are essential for defining the emergence of infection, determining the period that preventive measures should be applied, for evaluating drug and vaccine efficacy, and for controlling epidemics. In this study, we show that functionalized NPs conjugated to monoclonal antibodies can be used to rapidly and specifically detect respiratory syncytial virus in vitro and in vivo. These results suggest that functionalized NPs can provide direct, rapid, and sensitive detection of viruses and thereby bridge the gap between current cumbersome virus detection assays and the burgeoning need for more rapid and sensitive detection of viral agents.
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影响因子：
3.7
作者：
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通讯作者：
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影响因子：
46.9
作者：
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DOI：
10.1073/pnas.0509739103
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影响因子：
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通讯作者：
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作者：
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通讯作者：
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DOI：
10.1073/pnas.0601755103
发表时间：
2006-04-18
影响因子：
11.1
作者：
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通讯作者：
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