Enriched housing promotes post-stroke functional recovery through astrocytic HMGB1-IL-6-mediated angiogenesis.
Enriched housing promotes post-stroke functional recovery through astrocytic HMGB1-IL-6-mediated angiogenesis.
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丰富的住房通过星形细胞 HMGB1-IL-6 介导的血管生成促进中风后功能恢复。
DOI：
10.1038/cddiscovery.2017.54
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发表时间：
2017
影响因子：
7
通讯作者：
Yao SL
中科院分区：
文献类型：
--
作者：
Chen JY;Yu Y;Yuan Y;Zhang YJ;Fan XP;Yuan SY;Zhang JC;Yao SL
丰富的环境（EE）被证明可以促进缺血性卒中后的血管生成、神经发生和功能恢复。然而，其潜在机制仍不清楚。C57 BL/6小鼠大脑中动脉闭塞（60分钟），然后再灌注，然后将小鼠圈养在标准环境（SE）或EE。在这里，我们发现，缺血后EE表现出减少抑郁和焦虑样行为，并促进血管生成和功能恢复相比，SE小鼠。高迁移率族蛋白1（HMGB 1）抑制剂治疗的EE小鼠中风后抑郁和焦虑样行为增加，血管生成和功能恢复减少。EE小鼠星形胶质细胞HMGB 1和IL-6表达增加。用重组HMGB 1（rHMGB 1）处理的EE小鼠降低了IL-6和p-AKT的水平，而用重组HMGB 1（rHMGB 1）处理的EE小鼠增加了IL-6和p-AKT的水平。在EE小鼠中用抗IL-6中和抗体阻断IL-6可减弱EE介导的血管生成和功能恢复。此外，我们的体外数据显示，在原代星形胶质细胞培养中，rHMGB 1促进活化星形胶质细胞中IL-6的表达。PI 3 K/AKT信号通路参与HMGB 1介导的星形胶质细胞IL-6表达。因此，我们的研究结果揭示了一个以前未知的属性HMGB 1/IL-6信号通路在EE介导的血管生成和功能恢复缺血性中风后。
Enriched environment (EE) is shown to promote angiogenesis, neurogenesis and functional recovery after ischemic stroke. However, the underlying mechanisms remain unclear. C57BL/6 mice underwent middle cerebral artery occlusion (60 min) followed by reperfusion, after which mice were housed in either standard environment (SE) or EE. Here we found that post-ischemic EE exhibited decreased depression and anxiety-like behavior, and promoted angiogenesis and functional recovery compared to SE mice. EE mice treated with high-mobility group box-1 (HMGB1) inhibitor glycyrrhizin had an increased post-stroke depression and anxiety-like behavior, and the angiogenesis and functional recovery were decreased. HMGB1 and interleukin-6 (IL-6) expression in astrocyte were increased in EE mice. EE mice treated with glycyrrhizin decreased, whereas EE mice treated with recombinant HMGB1 (rHMGB1) increased the levels of IL-6 and p-AKT. Blockade of IL-6 with anti-IL-6-neutralizing antibody in EE mice attenuated EE-mediated angiogenesis and functional recovery. Furthermore, our in vitro data revealed that in primary astrocyte cultures rHMGB1 promoted the expression of IL-6 in activated astrocytes. PI3K/AKT signaling pathway was involved in HMGB1-mediated expression of astrocytic IL-6. Thus, our results reveal a previously uncharacterized property of HMGB1/IL-6 signaling pathway in EE-mediated angiogenesis and functional recovery after ischemic stroke.
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影响因子：
37.8
作者：
Go AS;Mozaffarian D;Roger VL;Benjamin EJ;Berry JD;Blaha MJ;Dai S;Ford ES;Fox CS;Franco S;Fullerton HJ;Gillespie C;Hailpern SM;Heit JA;Howard VJ;Huffman MD;Judd SE;Kissela BM;Kittner SJ;Lackland DT;Lichtman JH;Lisabeth LD;Mackey RH;Magid DJ;Marcus GM;Marelli A;Matchar DB;McGuire DK;Mohler ER 3rd;Moy CS;Mussolino ME;Neumar RW;Nichol G;Pandey DK;Paynter NP;Reeves MJ;Sorlie PD;Stein J;Towfighi A;Turan TN;Virani SS;Wong ND;Woo D;Turner MB;American Heart Association Statistics Committee and Stroke Statistics Subcommittee
通讯作者：
American Heart Association Statistics Committee and Stroke Statistics Subcommittee
影响因子：
8.3
作者：
KRUPINSKI, J;KALUZA, J;WANG, JM
通讯作者：
WANG, JM
DOI：
10.1073/pnas.1121146109
发表时间：
2012-05-08
影响因子：
11.1
作者：
Hayakawa, Kazuhide;Pham, Loc-Duyen D.;Lo, Eng H.
通讯作者：
Lo, Eng H.
影响因子：
14.5
作者：
Gertz, Karen;Kronenberg, Golo;Endres, Matthias
通讯作者：
Endres, Matthias
影响因子：
8.3
作者：
Komitova, M;Mattsson, B;Eriksson, PS
通讯作者：
Eriksson, PS