Intrathecal leptin inhibits expression of the P2X2/3 receptors and alleviates neuropathic pain induced by chronic constriction sciatic nerve injury.
Intrathecal leptin inhibits expression of the P2X2/3 receptors and alleviates neuropathic pain induced by chronic constriction sciatic nerve injury.
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鞘内注射瘦素抑制 P2X2/3 受体的表达并减轻慢性收缩性坐骨神经损伤引起的神经性疼痛
DOI：
10.1186/1744-8069-9-65
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发表时间：
2013-12-10
期刊：
影响因子：
3.3
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Li X;Kang L;Li G;Zeng H;Zhang L;Ling X;Dong H;Liang S;Chen H
研究背景瘦素是一种主要由白色脂肪组织产生的脂肪细胞因子，在神经功能调节中具有广泛的作用。越来越多的证据表明，瘦素在影响神经病理性疼痛中起着重要作用，最近的发现表明，长期给予瘦素诱导了幼稚大鼠的热痛觉过敏和机械异常性疼痛。坐骨神经慢性压迫性损伤（CCI）是一种用于研究神经病理性疼痛的良好模型。本研究旨在通过检查特定的疼痛行为来探讨瘦素是否在CCI诱导的大鼠神经病理性疼痛中发挥作用。结果大鼠坐骨神经损伤后，同侧背根神经节（DRG）内源性瘦素及瘦素受体（OB-Rb）水平呈时间依赖性升高。CCI后7天开始鞘内注射瘦素，每天1次，持续6天，可减轻神经病理性疼痛，并降低IL-6、TNFα和P2 X2和P2 X3受体的表达。鞘内注射OB-Rb反义寡核苷酸减弱DRG内源性OB-Rb并不改变CCI诱导的热痛觉过敏或机械性异常性疼痛。结论外源性瘦素可减轻CCI所致的慢性神经病理性疼痛。瘦素的作用可能是通过抑制CCI大鼠背根节中IL-6、TNFα和P2 X2、P2 X3受体的表达而介导的。
Background Leptin, an adipocytokine produced mainly by white adipose tissue, has a broad role in the regulation of neuronal functions. Accumulating evidence has revealed that leptin plays an important role in influencing neuropathic pain, shown recently by the finding that chronic administration of leptin induced thermal hyperalgesia and mechanical allodynia in naïve rats. Chronic constriction sciatic nerve injury (CCI) is a well characterized model used for studying neuropathic pain. The present study was designed to investigate whether leptin plays a role in neuropathic pain in rats induced by CCI by examining particular pain behaviors. Results After sciatic nerve injury in rats, endogenous levels of leptin and leptin receptor (OB-Rb) were increased in a time dependent manner within the ipsilateral dorsal root ganglion (DRG). Intrathecal administration of leptin once daily for 6 days, beginning 7 days after CCI, alleviated neuropathic pain and decreased the expression of IL-6, TNFα, and the P2X2 and P2X3 receptors. Attenuation of endogenous OB-Rb in the DRG by intrathecal administration of OB-Rb antisense oligonucleotides did not change thermal hyperalgesia or mechanical allodynia induced by CCI. Conclusions Our findings suggest that exogenous leptin can alleviate the chronic neuropathic pain caused by CCI. The leptin effect may be mediated by attenuated expression of IL-6, TNFα, and the P2X2 and P2X3 receptors in the DRG of CCI rats.
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影响因子：
3.3
作者：
Quarta S;Vogl C;Constantin CE;Üçeyler N;Sommer C;Kress M
通讯作者：
Kress M
影响因子：
9.3
作者：
Dubový P;Brázda V;Klusáková I;Hradilová-Svíženská I
通讯作者：
Hradilová-Svíženská I
影响因子：
3.3
作者：
Sakurai E;Kurihara T;Kouchi K;Saegusa H;Zong S;Tanabe T
通讯作者：
Tanabe T
影响因子：
3.8
作者：
Gao, Yun;Liu, Han;Liang, Shangdong
通讯作者：
Liang, Shangdong
DOI：
10.1006/bbrc.1999.0382
发表时间：
1999-03-24
影响因子：
3.1
作者：
Steppan, CM;Swick, AG
通讯作者：
Swick, AG