Sertoli cell-enriched proteins in mouse and human testicular interstitial fluid.
Sertoli cell-enriched proteins in mouse and human testicular interstitial fluid.
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DOI：
10.1371/journal.pone.0290846
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发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
支持细胞支持精子的发育以及小管间质中各种体细胞的功能。支持细胞调节睾丸脉管系统的功能以及产生睾酮以促进生育和男子气概的间质细胞的发育和功能。然而，调节这些细胞的支持细胞衍生因子在很大程度上是未知的。为了确定支持细胞支持睾丸体细胞功能的潜在机制，我们的目的是鉴定小管之间睾丸间质液（TIF）中富含支持细胞的蛋白质。我们之前解析了小鼠和人类中 TIF 的蛋白质组，并证明它是生精小管衍生蛋白质的丰富来源。在当前的研究中，我们设计了生物信息学策略来询问相关的蛋白质组和基因组数据集，以识别小鼠和人类 TIF 中富含支持细胞的蛋白质。我们分析了小鼠 TIF 中的蛋白质，这些蛋白质在体内急性支持细胞消融一周后显着减少，并根据相关的小鼠睾丸 RNASeq 数据集验证了其中哪些可能主要来自支持细胞。我们利用高质量的人类睾丸基因组、蛋白质组和免疫组织化学数据集，使用不同但互补的方法来鉴定人类 TIF 中富含支持细胞的蛋白质。我们在小鼠和人类 TIF 中分别鉴定了总共 47 种和 40 种富含支持细胞的蛋白质，其中包括 15 种在这两个物种中保守的蛋白质。鉴定出在血管生成、间质细胞或类固醇生成以及免疫细胞调节中具有潜在作用的蛋白质。数据表明，其中一些蛋白质是分泌的，但支持细胞也通过释放细胞外囊泡将特定蛋白质沉积到 TIF 中。总之，我们在 TIF 中鉴定出了富含支持细胞的新型蛋白，它们是调节体细胞间通讯和睾丸功能的候选蛋白。
Sertoli cells support the development of sperm and the function of various somatic cells in the interstitium between the tubules. Sertoli cells regulate the function of the testicular vasculature and the development and function of the Leydig cells that produce testosterone for fertility and virility. However, the Sertoli cell-derived factors that regulate these cells are largely unknown. To define potential mechanisms by which Sertoli cells could support testicular somatic cell function, we aimed to identify Sertoli cell-enriched proteins in the testicular interstitial fluid (TIF) between the tubules. We previously resolved the proteome of TIF in mice and humans and have shown it to be a rich source of seminiferous tubule-derived proteins. In the current study, we designed bioinformatic strategies to interrogate relevant proteomic and genomic datasets to identify Sertoli cell-enriched proteins in mouse and human TIF. We analysed proteins in mouse TIF that were significantly reduced after one week of acute Sertoli cell ablation in vivo and validated which of these are likely to arise primarily from Sertoli cells based on relevant mouse testis RNASeq datasets. We used a different, but complementary, approach to identify Sertoli cell-enriched proteins in human TIF, taking advantage of high-quality human testis genomic, proteomic and immunohistochemical datasets. We identified a total of 47 and 40 Sertoli cell-enriched proteins in mouse and human TIF, respectively, including 15 proteins that are conserved in both species. Proteins with potential roles in angiogenesis, the regulation of Leydig cells or steroidogenesis, and immune cell regulation were identified. The data suggests that some of these proteins are secreted, but that Sertoli cells also deposit specific proteins into TIF via the release of extracellular vesicles. In conclusion, we have identified novel Sertoli cell-enriched proteins in TIF that are candidates for regulating somatic cell-cell communication and testis function.
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影响因子：
4.8
作者：
Rebourcet D;Darbey A;Monteiro A;Soffientini U;Tsai YT;Handel I;Pitetti JL;Nef S;Smith LB;O'Shaughnessy PJ
通讯作者：
O'Shaughnessy PJ
影响因子：
2.5
作者：
Houston, Brendan J.;Nagirnaja, Liina;Merriner, D. Jo;O'Connor, Anne E.;Okuda, Hidenobu;Omurtag, Kenan;Smith, Craig;Aston, Kenneth I.;Conrad, Donald F.;O'Bryan, Moira K.
通讯作者：
O'Bryan, Moira K.
影响因子：
44.1
作者：
Guo J;Grow EJ;Mlcochova H;Maher GJ;Lindskog C;Nie X;Guo Y;Takei Y;Yun J;Cai L;Kim R;Carrell DT;Goriely A;Hotaling JM;Cairns BR
通讯作者：
Cairns BR
影响因子：
3.6
作者：
Chen, Min;Wang, Xiaona;Gao, Fei
通讯作者：
Gao, Fei
影响因子：
3.6
作者：
Guttman, JA;Takai, Y;Vogl, AW
通讯作者：
Vogl, AW