A Self-Assembling Injectable Biomimetic Microenvironment Encourages Retinal Ganglion Cell Axon Extension in Vitro.
A Self-Assembling Injectable Biomimetic Microenvironment Encourages Retinal Ganglion Cell Axon Extension in Vitro.
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DOI：
10.1021/acsami.6b04679
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发表时间：
2016-08-17
影响因子：
9.5
通讯作者：
Park D
中科院分区：
文献类型：
--
作者：
Laughter MR;Ammar DA;Bardill JR;Pena B;Kahook MY;Lee DJ;Park D
感觉-躯体神经系统神经元，如视网膜神经节细胞(RGC)，通常被认为不能再生。然而，现在已经知道，通过为这些细胞提供生长允许的环境，可以刺激它们再生。我们设计了一个可注射的微环境，旨在为RGC培养提供生长刺激线索。这种可注射材料在凝胶化后不仅会自我组装成层状薄片，类似于视网膜组织，而且还具有与视网膜组织相当的存储模数。原代培养的大鼠视网膜神经节细胞在这种三维支架上进行培养、染色和成像。我们能够证明，生长在这种视网膜样结构中的RGC表现出特有的长而突出的轴突。此外，培养7天后，RGCs的平均轴突长度和轴突负载率持续增加，表明该支架能够支持RGC轴突的显著延长。
Sensory-somatic nervous system neurons, such as retinal ganglion cells (RGCs), are typically thought to be incapable of regenerating. However, it is now known that these cells may be stimulated to regenerate by providing them with a growth permissive environment. We have engineered an injectable microenvironment designed to provide growth-stimulating cues for RGC culture. Upon gelation, this injectable material not only self-assembles into laminar sheets, similar to retinal organization, but also possesses a storage modulus comparable to that of retinal tissue. Primary rat RGCs were grown, stained, and imaged in this three-dimensional scaffold. We were able to show that RGCs grown in this retina-like structure exhibited characteristic long, prominent axons. In addition, RGCs showed a consistent increase in average axon length and neurite-bearing ratio over the 7 day culture period, indicating this scaffold is capable of supporting substantial RGC axon extension.
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影响因子：
14
作者：
Kador, Karl E.;Montero, Ramon B.;Venugopalan, Praseeda;Hertz, Jonathan;Zindell, Allison N.;Valenzuela, Daniel A.;Uddin, Mohammed S.;Lavik, Erin B.;Muller, Kenneth J.;Andreopoulos, Fotios M.;Goldberg, Jeffrey L.
通讯作者：
Goldberg, Jeffrey L.
影响因子：
3.9
作者：
Morgan, JE
通讯作者：
Morgan, JE
DOI：
10.1073/pnas.0606150103
发表时间：
2006-11-21
影响因子：
11.1
作者：
Lu, Yun-Bi;Franze, Kristian;Reichenbach, Andreas
通讯作者：
Reichenbach, Andreas
影响因子：
3.5
作者：
Jung, M;Petrausch, B;Stuermer, CAO
通讯作者：
Stuermer, CAO
影响因子：
4.9
作者：
Mazumder, M. A. Jafar;Fitzpatrick, Scott D.;Sheardown, Heather
通讯作者：
Sheardown, Heather