Vertical variations in microbial diversity, composition, and interactions in freshwater lake sediments on the Tibetan plateau.
Vertical variations in microbial diversity, composition, and interactions in freshwater lake sediments on the Tibetan plateau.
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DOI：
10.3389/fmicb.2023.1118892
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
淡水湖沉积物中的微生物群落表现出明显的深度依赖性变异性。需要进一步探索以了解其生物多样性模式和垂直沉积物中的微生物相互作用。在这项研究中，对青藏高原木格措（MGC）和措普（CP）两个淡水湖的沉积物岩心进行了采样，然后将其切成每厘米或半厘米深度的层。扩增子测序用于分析微生物群落的组成、多样性和相互作用。结果表明，两个湖泊的沉积物样品在沉积深度约20cm处可聚集成两组，微生物群落组成发生明显变化。在MGC湖中，丰富度成分以β多样性为主，并且随着深度的增加而增加，表明MGC深层的微生物群落是从表层选择出来的。相反，替换成分在CP中的β多样性中占主导地位，这意味着表层的周转率较高，而深层的种子库不活跃，多样性较高。共现网络分析表明，负性微生物相互作用在营养物浓度高的表层普遍存在，而正性微生物相互作用在营养物浓度低的深层更常见，这表明微生物相互作用受到垂直沉积物营养条件的影响。此外，结果强调了丰富和稀有类群分别对微生物相互作用和 β 多样性垂直波动的显着贡献。总体而言，这项工作加深了我们对湖泊沉积物柱中微生物相互作用和β多样性垂直波动模式的理解，特别是青藏高原的淡水湖泊沉积物。
Microbial communities in freshwater lake sediments exhibit a distinct depth-dependent variability. Further exploration is required to understand their biodiversity pattern and microbial interactions in vertical sediments. In this study, sediment cores from two freshwater lakes, Mugecuo (MGC) and Cuopu (CP), on the Tibetan plateau were sampled and subsequently sliced into layers at a depth of every centimeter or half a centimeter. Amplicon sequencing was used to analyze the composition, diversity, and interaction of microbial communities. Results showed that sediment samples of both lakes could be clustered into two groups at a sediment depth of about 20 cm, with obvious shifts in microbial community compositions. In lake MGC, the richness component dominated β-diversity and increased with depth, indicating that the microbial communities in the deep layer of MGC was selected from the surface layer. Conversely, the replacement component dominated β-diversity in CP, implying a high turnover rate in the surface layer and inactive seed banks with a high variety in the deep layer. A co-occurrence network analysis showed that negative microbial interactions were prevalent in the surface layers with high nutrient concentrations, while positive microbial interactions were more common in the deep layers with low nutrient concentrations, suggesting that microbial interactions are influenced by nutrient conditions in the vertical sediments. Additionally, the results highlight the significant contributions of abundant and rare taxa to microbial interactions and vertical fluctuations of β-diversity, respectively. Overall, this work deepens our understanding of patterns of microbial interactions and vertical fluctuation in β-diversity in lake sediment columns, particularly in freshwater lake sediments from the Tibetan plateau.
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影响因子：
4.4
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