Profiling of chromatin accessibility identifies transcription factor binding sites across the genome of Aspergillus species.
Profiling of chromatin accessibility identifies transcription factor binding sites across the genome of Aspergillus species.
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染色质可及性分析确定了曲霉属物种基因组中的转录因子结合位点
DOI：
10.1186/s12915-021-01114-0
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发表时间：
2021-09-06
期刊：
影响因子：
5.4
通讯作者：
Pan L
中科院分区：
文献类型：
--
作者：
Huang L;Li X;Dong L;Wang B;Pan L
背景
Background
The identification of open chromatin regions and transcription factor binding sites (TFBs) is an important step in understanding the regulation of gene expression in diverse species. ATAC-seq is a technique used for such purpose by providing high-resolution measurements of chromatin accessibility revealed through integration of Tn5 transposase. However, the existence of cell walls in filamentous fungi and associated difficulty in purifying nuclei have precluded the routine application of this technique, leading to a lack of experimentally determined and computationally inferred data on the identity of genome-wide cis-regulatory elements (CREs) and TFBs. In this study, we constructed an ATAC-seq platform suitable for filamentous fungi and generated ATAC-seq libraries of Aspergillus niger and Aspergillus oryzae grown under a variety of conditions.
Results
We applied the ATAC-seq assay for filamentous fungi to delineate the syntenic orthologue and differentially changed chromatin accessibility regions among different Aspergillus species, during different culture conditions, and among specific TF-deleted strains. The syntenic orthologues of accessible regions were responsible for the conservative functions across Aspergillus species, while regions differentially changed between culture conditions and TFs mutants drove differential gene expression programs. Importantly, we suggest criteria to determine TFBs through the analysis of unbalanced cleavage of distinct TF-bound DNA strands by Tn5 transposase. Based on this criterion, we constructed data libraries of the in vivo genomic footprint of A. niger under distinct conditions, and generated a database of novel transcription factor binding motifs through comparison of footprints in TF-deleted strains. Furthermore, we validated the novel TFBs in vivo through an artificial synthetic minimal promoter system.
Conclusions
We characterized the chromatin accessibility regions of filamentous fungi species, and identified a complete TFBs map by ATAC-seq, which provides valuable data for future analyses of transcriptional regulation in filamentous fungi.
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影响因子：
4.4
作者：
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通讯作者：
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影响因子：
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影响因子：
5.6
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通讯作者：
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影响因子：
1.6
作者：
Dong, Liangbo;Yu, Dou;Pan, Li
通讯作者：
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影响因子：
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通讯作者：
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