Depression of rat testicular 17-hydroxylase and 17,20-lyase after administration of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).
Depression of rat testicular 17-hydroxylase and 17,20-lyase after administration of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).
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给予 2,3,7,8-四氯二苯并-对二恶英 (TCDD) 后大鼠睾丸 17-羟化酶和 17,20-裂解酶降低。
DOI：
10.1016/0006-2952(87)90726-x
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发表时间：
1987
影响因子：
5.8
通讯作者：
Piper,WN
中科院分区：
文献类型：
--
作者：
Mebus,CA;Reddy,VR;Piper,WN
2，3，7，8-四氯二苯并-对二恶英(TCDD)的毒性作用，如氯化痤疮、多毛症、皮肤色素沉着等提示内分泌参与，但TCDD对类固醇合成器官的影响知之甚少。已知TCDD可导致大鼠睾丸血红素、睾丸微粒体细胞色素P-450和血清睾酮水平下降。本研究旨在研究口服12.5、25或50μg/kg TCDD12.5、25或50μg/kg TCDD12.5、25或50 mg/kgTCDD对小鼠睾丸血色素P450依赖酶17-羟基酶和17，20-裂解酶活性的影响。TCDD使17-羟基酶活性呈剂量和时间依赖性下降。最低剂量12.5μg/kgTCDD在第3、7、14天显著降低。17，20-裂解酶对TCDD的毒性反应较弱，仅在50μg/kg TCDD处理后的第3、7和14天，酶活性才显著降低。12.5、2 5和5 0μg/kg剂量组细胞色素P 4 5 0和血清睾酮水平均呈剂量和时间依赖性下降。这些结果表明，TCDD处理后睾酮的产生减少与睾丸微粒体细胞色素P-450依赖的酶17-羟基酶和17，20-裂解酶活性降低有关。
Toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), such as chloracne, hirsutism, and skin hyperpigmentation, suggest endocrine involvement, however, little is known about the effects of TCDD on steroidogenic organs. It is known that TCDD can cause decreases in testicular heme, testicular microsomal cytochrome P-450, and serum testosterone in the rat. This study was designed to examine the activities of the testicular hemoprotein microsomal cytochrome P-450-dependent enzymes, 17-hydroxylase and 17,20-lyase, following a single, oral dose of either 12.5, 25, or 50 μg/kg TCDD. TCDD caused dose- and time-dependent decreases in the activity of the 17-hydroxylase enzyme. Significant decreases were observed at 3, 7 and 14 days at the lowest dose of 12.5 μg/kg TCDD. The 17,20-lyase enzyme seemed to be less sensitive to the toxic effects of TCDD with significant decreases in enzyme activity being observed at days 3, 7 and 14 only after treatment with 50 μg/kg TCDD. Both microsomal cytochrome P-450 and serum testosterone levels decreased in a dose- and time-dependent manner following 12.5, 25 and 50 μg/kg doses of TCDD. These results indicate that decreased testosterone production following treatment with TCDD is related to decreased activities of the testicular microsomal cytochrome P-450-dependent enzymes 17-hydroxylase and 17,20-lyase.
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DOI：
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