Fully resolved assembly of Cryptosporidium parvum.
Fully resolved assembly of Cryptosporidium parvum.
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DOI：
10.1093/gigascience/giac010
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发表时间：
2022-02-15
期刊：
影响因子：
9.2
通讯作者：
Sedlazeck FJ
中科院分区：
文献类型：
--
作者：
Menon VK;Okhuysen PC;Chappell CL;Mahmoud M;Mahmoud M;Meng Q;Doddapaneni H;Vee V;Han Y;Salvi S;Bhamidipati S;Kottapalli K;Weissenberger G;Shen H;Ross MC;Hoffman KL;Cregeen SJ;Muzny DM;Metcalf GA;Gibbs RA;Petrosino JF;Sedlazeck FJ
Cryptosporidium parvum is an apicomplexan parasite commonly found across many host species with a global infection prevalence in human populations of 7.6%. Understanding its diversity and genomic makeup can help in fighting established infections and prohibiting further transmission. The basis of every genomic study is a high-quality reference genome that has continuity and completeness, thus enabling comprehensive comparative studies. Here, we provide a highly accurate and complete reference genome of Cryptosporidium parvum. The assembly is based on Oxford Nanopore reads and was improved using Illumina reads for error correction. We also outline how to evaluate and choose from different assembly methods based on 2 main approaches that can be applied to other Cryptosporidium species. The assembly encompasses 8 chromosomes and includes 13 telomeres that were resolved. Overall, the assembly shows a high completion rate with 98.4% single-copy BUSCO genes. This high-quality reference genome of a zoonotic IIaA17G2R1 C. parvum subtype isolate provides the basis for subsequent comparative genomic studies across the Cryptosporidium clade. This will enable improved understanding of diversity, functional, and association studies.
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DOI：
10.1038/s41576-021-00367-3
发表时间：
2021-09
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
De Coster W;Weissensteiner MH;Sedlazeck FJ
通讯作者：
Sedlazeck FJ
DOI：
10.1016/s2214-109x(20)30357-0
发表时间：
2020-10
期刊：
The Lancet. Global health
影响因子：
--
作者：
Head MG;Brown RJ;Newell ML;Scott JAG;Batchelor J;Atun R
通讯作者：
Atun R
影响因子：
3.2
作者：
Guy RA;Yanta CA;Muchaal PK;Rankin MA;Thivierge K;Lau R;Boggild AK
通讯作者：
Boggild AK
影响因子：
168.9
作者：
Kotloff, Karen L.;Nataro, James P.;Levine, Myron M.
通讯作者：
Levine, Myron M.
影响因子：
48
作者：
Chin, Chen-Shan;Peluso, Paul;Schatz, Michael C.
通讯作者：
Schatz, Michael C.