Molecular characterization of Cryptosporidium isolates from humans in Ontario, Canada.
Molecular characterization of Cryptosporidium isolates from humans in Ontario, Canada.
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DOI：
10.1186/s13071-020-04546-9
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发表时间：
2021-01-22
影响因子：
3.2
通讯作者：
Boggild AK
中科院分区：
文献类型：
--
作者：
Guy RA;Yanta CA;Muchaal PK;Rankin MA;Thivierge K;Lau R;Boggild AK
隐孢子虫病是一种全球性分布的胃肠道疾病。自2000年以来，它在加拿大一直是一种可报告的疾病；然而，没有进行常规的分子监测。因此，污染源是未知的。该项目的目的是确定加拿大最大省份安大略省临床病例中隐孢子虫的种类和亚型，以了解传播模式。安大略省是加拿大最大的省份，占加拿大人口的三分之一。使用分子工具对2008-2017年间镜检呈隐孢子虫阳性的169份冷冻、储存、未保存的粪便样本进行了鉴定。169个样本中的一个子集是来自个别病例的复制样本。直接从粪便中提取DNA，套式聚合酶链式反应，然后针对小亚单位核糖体RNA(SSU)和糖蛋白60(Gp60)基因进行Sanger测序。获得129例隐孢子虫病的分子分型数据和有限的人口学数据。其中微小隐孢子虫91例(70.5%)，人隐孢子虫24例(18.6%)。4例(3.1%)出现微小弧菌和人弧菌混合感染。其他5种为泛隐孢子虫(n=5)、费氏隐孢子虫(n=2)、梅氏隐孢子虫(n=1)、楔形隐孢子虫(n=1)和小鼠隐孢子虫(n=1)。对gp60基因进行亚型分型，发现了5个等位基因家系和17个亚型，包括3个等位基因家族和17个亚型。人毛滴虫最常见的亚型是IbA10G2(22.3%)，微小毛滴虫的亚型是IIaA15G2R1(62.4%)。本研究中的大多数分离株为微小隐孢子虫，支持人畜共患传播是安大略省隐孢子虫病传播的主要途径的观点。尽管如此，在大约四分之一的病例中观察到的人间隐孢子虫表明，人间传播也是安大略省隐孢子虫病发病的一个重要因素。
Cryptosporidiosis is a gastrointestinal disease with global distribution. It has been a reportable disease in Canada since 2000; however, routine molecular surveillance is not conducted. Therefore, sources of contamination are unknown. The aim of this project was to identify species and subtypes of Cryptosporidium in clinical cases from Ontario, the largest province in Canada, representing one third of the Canadian population, in order to understand transmission patterns. A total of 169 frozen, banked, unpreserved stool specimens that were microscopy positive for Cryptosporidium over the period 2008–2017 were characterized using molecular tools. A subset of the 169 specimens were replicate samples from individual cases. DNA was extracted directly from the stool and nested PCR followed by Sanger sequencing was conducted targeting the small subunit ribosomal RNA (SSU) and glycoprotein 60 (gp60) genes. Molecular typing data and limited demographic data were obtained for 129 cases of cryptosporidiosis. Of these cases, 91 (70.5 %) were due to Cryptosporidium parvum and 24 (18.6%) were due to Cryptosporidium hominis. Mixed infections of C. parvum and C. hominis occurred in four (3.1%) cases. Five other species observed were Cryptosporidium ubiquitum (n = 5), Cryptosporidium felis (n = 2), Cryptosporidium meleagridis (n = 1), Cryptosporidium cuniculus (n = 1) and Cryptosporidium muris (n = 1). Subtyping the gp60 gene revealed 5 allelic families and 17 subtypes of C. hominis and 3 allelic families and 17 subtypes of C. parvum. The most frequent subtype of C. hominis was IbA10G2 (22.3%) and of C. parvum was IIaA15G2R1 (62.4%). The majority of isolates in this study were C. parvum, supporting the notion that zoonotic transmission is the main route of cryptosporidiosis transmission in Ontario. Nonetheless, the observation of C. hominis in about a quarter of cases suggests that anthroponotic transmission is also an important contributor to cryptosporidiosis pathogenesis in Ontario.
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DOI：
10.15585/mmwr.mm6619a2
发表时间：
2017-05-19
期刊：
MMWR. Morbidity and mortality weekly report
影响因子：
--
作者：
Hlavsa MC;Roellig DM;Seabolt MH;Kahler AM;Murphy JL;McKitt TK;Geeter EF;Dawsey R;Davidson SL;Kim TN;Tucker TH;Iverson SA;Garrett B;Fowle N;Collins J;Epperson G;Zusy S;Weiss JR;Komatsu K;Rodriguez E;Patterson JG;Sunenshine R;Taylor B;Cibulskas K;Denny L;Omura K;Tsorin B;Fullerton KE;Xiao L
通讯作者：
Xiao L
影响因子：
11.8
作者：
Li N;Xiao L;Alderisio K;Elwin K;Cebelinski E;Chalmers R;Santin M;Fayer R;Kvac M;Ryan U;Sak B;Stanko M;Guo Y;Wang L;Zhang L;Cai J;Roellig D;Feng Y
通讯作者：
Feng Y
5岁以下儿童的腹泻感染与腹泻有关的发病率，死亡率和长期后果：一项荟萃分析研究。
DOI：
10.1016/s2214-109x(18)30283-3
发表时间：
2018-07
期刊：
The Lancet. Global health
影响因子：
--
作者：
Khalil IA;Troeger C;Rao PC;Blacker BF;Brown A;Brewer TG;Colombara DV;De Hostos EL;Engmann C;Guerrant RL;Haque R;Houpt ER;Kang G;Korpe PS;Kotloff KL;Lima AAM;Petri WA Jr;Platts-Mills JA;Shoultz DA;Forouzanfar MH;Hay SI;Reiner RC Jr;Mokdad AH
通讯作者：
Mokdad AH
影响因子：
34.3
作者：
Kotloff, Karen L.;Nasrin, Dilruba;Levine, Myron M.
通讯作者：
Levine, Myron M.
影响因子：
11.8
作者：
Chalmers, Rachel M.;Hadfield, Stephen J.;Hunter, Paul
通讯作者：
Hunter, Paul