Immune profiling of BALB/C and C57BL/6 mice reveals a correlation between Ureaplasma parvum-Induced fetal inflammatory response syndrome-like pathology and increased placental expression of TLR2 and CD14.
Immune profiling of BALB/C and C57BL/6 mice reveals a correlation between Ureaplasma parvum-Induced fetal inflammatory response syndrome-like pathology and increased placental expression of TLR2 and CD14.
复制标题
DOI：
10.1111/aji.12192
复制
发表时间：
2014-03
期刊：
影响因子：
--
通讯作者：
Reyes L
中科院分区：
文献类型：
--
作者：
Allam AB;von Chamier M;Brown MB;Reyes L
BALB/c和C57BL/6小鼠都对微小解脲原体宫内感染易感，但只有典型的TH2/M2 BALB/c小鼠出现严重的绒毛膜羊膜炎、胎儿感染和胎儿炎症反应综合征样(FIFS)病理。用显微镜、基因表达分析和酶联免疫吸附试验鉴定与巨噬细胞极性、严重绒毛膜羊膜炎和胎儿感染相关的胎盘先天免疫反应。这两个品系的小鼠在母体-胎儿界面上都表现出前M2细胞因子的特征。BALB/c小鼠感染胎盘中CD14和TLR1、2、6表达增加，TLR2和CD14定位于中性粒细胞。在有FIRS病理证据的组织中，BALB/c合体滋养层细胞的TLR2/CD14也升高。相比之下，C57BL/6胎盘中的表达要么没有变化，要么下调。我们的发现表明，BALB/c小鼠合体滋养层细胞CD14/TLR2表达增加与FIRS样病理之间存在联系。需要进行功能研究，以确定CD14是否导致绒毛膜羊膜炎期间胎儿发病率的增加。
Both BALB/c and C57BL/6 mice are susceptible to intrauterine infection with U. parvum, but only protypical TH2/M2 BALB/c mice develop severe chorioamnionitis, fetal infection, and fetal inflammatory response syndrome-like (FIRS) pathology. Microscopy, gene expression analysis, and ELISA were used to identify placental innate immune responses relevant to macrophage polarity, severe chorioamnionitis, and fetal infection. Both mouse strains exhibited a pro-M2 cytokine profile at the maternal-fetal interface. In BALB/c mice, expression of CD14 and TLRs 1, 2, 6 was increased in infected placentas; TLR2 and CD14 were localized to neutrophils. Increased TLR2/CD14 was also observed in BALB/c syncytiotrophoblasts in tissues with pathological evidence of FIRS. In contrast, expression in C57BL/6 placentas was either unchanged or down-regulated. Our findings show a link between increased syncytiotrophoblast expression of CD14/TLR2 and FIRS-like pathology in BALB/c mice. Functional studies are required to determine if CD14 is contributing to fetal morbidity during chorioamnionitis.
登录
查看更多内容
影响因子：
64.8
作者：
Hoebe, K;Georgel, P;Beutler, B
通讯作者：
Beutler, B
影响因子：
3.7
作者：
Allam AB;Alvarez S;Brown MB;Reyes L
通讯作者：
Reyes L
影响因子：
3.8
作者：
Chaudhuri, S.;Lowen, B.;Guilbert, L. J.
通讯作者：
Guilbert, L. J.
影响因子：
3.9
作者：
Buhimschi CS;Bhandari V;Han YW;Dulay AT;Baumbusch MA;Madri JA;Buhimschi IA
通讯作者：
Buhimschi IA
影响因子：
6.4
作者：
Gerber, S;Vial, Y;Witkin, SS
通讯作者：
Witkin, SS