Conserved amino acid sequence features in the alpha subunits of MoFe, VFe, and FeFe nitrogenases.
Conserved amino acid sequence features in the alpha subunits of MoFe, VFe, and FeFe nitrogenases.
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DOI：
10.1371/journal.pone.0006136
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发表时间：
2009-07-03
期刊：
影响因子：
3.7
通讯作者：
Kechris KJ
中科院分区：
文献类型：
--
作者：
Glazer AN;Kechris KJ
本研究分析了69个全长NifD（MoFe亚基）、VnfD（VFe亚基）和AnfD（FeFe亚基）α亚基序列的结构特征和同源性。这组序列的分析包括BLAST评分、多序列比对、协变残基模式检查、系统发育分析以及保守Cys和His残基侧翼序列的比较，这些残基将FeMo辅因子连接到NifD上，并且在替代性固氮酶中也是保守的。结果表明，NifD固氮酶分为两个不同的群体。组I包括来自细菌内的许多属的NifD序列，包括所检查的所有固氮需氧菌，以及严格厌氧菌和一些兼性厌氧菌，但没有古菌序列。相比之下，II组NifD序列仅限于少量严格厌氧菌的古细菌和细菌序列。VnfD和AnfD序列分为两个不同的组，与II组NifD的关系比与I组NifD的关系更密切。沿着组I和II NifD、VnfD和AnfD的全长分布的完全保守的残基的模式明确地证实了这些多肽衍生自共同的祖先序列。没有迹象表明，特定于上述四个组的协变残基的模式之间的关系，这将给出从一种类型的固氮酶到另一种类型的进化途径的指示。相反，数据的总体，沿着系统发育分析，与来自共同祖先序列的I组和II组NifD、VnfD和AnfD的辐射一致。这里提供的所有数据都有力地支持了一些早期研究者提出的建议，即固氮酶家族已经在古细菌和细菌的最后一个共同祖先中进化出来。
This study examines the structural features and phylogeny of the α subunits of 69 full-length NifD (MoFe subunit), VnfD (VFe subunit), and AnfD (FeFe subunit) sequences. The analyses of this set of sequences included BLAST scores, multiple sequence alignment, examination of patterns of covariant residues, phylogenetic analysis and comparison of the sequences flanking the conserved Cys and His residues that attach the FeMo cofactor to NifD and that are also conserved in the alternative nitrogenases. The results show that NifD nitrogenases fall into two distinct groups. Group I includes NifD sequences from many genera within Bacteria, including all nitrogen-fixing aerobes examined, as well as strict anaerobes and some facultative anaerobes, but no archaeal sequences. In contrast, Group II NifD sequences were limited to a small number of archaeal and bacterial sequences from strict anaerobes. The VnfD and AnfD sequences fall into two separate groups, more closely related to Group II NifD than to Group I NifD. The pattern of perfectly conserved residues, distributed along the full length of the Group I and II NifD, VnfD, and AnfD, confirms unambiguously that these polypeptides are derived from a common ancestral sequence. There is no indication of a relationship between the patterns of covariant residues specific to each of the four groups discussed above that would give indications of an evolutionary pathway leading from one type of nitrogenase to another. Rather the totality of the data, along with the phylogenetic analysis, is consistent with a radiation of Group I and II NifDs, VnfD and AnfD from a common ancestral sequence. All the data presented here strongly support the suggestion made by some earlier investigators that the nitrogenase family had already evolved in the last common ancestor of the Archaea and Bacteria.
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DOI：
10.1098/rsta.2004.1541
发表时间：
2005-04-15
影响因子：
5
作者：
Schrock, RR
通讯作者：
Schrock, RR
DOI：
10.1073/pnas.0711093105
发表时间：
2008-02-12
影响因子：
11.1
作者：
Seedorf, Henning;Fricke, W. Florian;Gottschalk, Gerhard
通讯作者：
Gottschalk, Gerhard
影响因子：
56.9
作者：
Einsle, O;Tezcan, FA;Rees, DC
通讯作者：
Rees, DC
DOI：
10.1098/rsta.2004.1539
发表时间：
2005-04-15
影响因子：
5
作者：
Rees, DC;Tezcan, FA;Howard, JB
通讯作者：
Howard, JB
影响因子：
2.8
作者：
Boison, Gudrun;Steingen, Caroline;Bothe, Hermann
通讯作者：
Bothe, Hermann