Efficiency of cell-type specific and generic promoters in transducing oxytocin neurons and monitoring their neural activity during lactation.
Efficiency of cell-type specific and generic promoters in transducing oxytocin neurons and monitoring their neural activity during lactation.
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DOI：
10.1038/s41598-021-01818-x
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发表时间：
2021-11-18
影响因子：
4.6
通讯作者：
Harony-Nicolas H
中科院分区：
文献类型：
--
作者：
Thirtamara Rajamani K;Leithead AB;Kim M;Barbier M;Peruggia M;Niblo K;Barteczko L;Lefevre A;Grinevich V;Harony-Nicolas H
下丘脑催产素（OXT）和精氨酸加压素（AVP）神经元一直是一些生理和行为研究的中心。病毒载体生物学的进步和转基因啮齿动物模型的发展允许靶向基因表达，以研究特定细胞群和脑回路的功能。在这项研究中，我们比较了各种腺相关病毒载体在这些细胞群中的效率，并证明了没有一种广泛使用的启动子本身能够有效地驱动OXT或AVP神经元中下游荧光蛋白的表达。如预期的，OXT启动子可以有效地驱动OXT神经元中的基因表达，并且这种效率仅归因于启动子而不是病毒血清型。我们还报道了使用OXT启动子驱动的Cre重组酶的双病毒方法显著提高了OXT神经元中病毒转导的效率。最后，我们证明了OXT启动子的效用进行OXT神经元的功能研究，通过使用OXT特异性病毒系统记录哺乳期雌性大鼠的OXT神经元的神经活动，随着时间的推移。我们的结论是，当采用非神经元特异性的病毒方法/启动子研究下丘脑室旁核内的神经种群时，需要非常谨慎。
Hypothalamic oxytocin (OXT) and arginine-vasopressin (AVP) neurons have been at the center of several physiological and behavioral studies. Advances in viral vector biology and the development of transgenic rodent models have allowed for targeted gene expression to study the functions of specific cell populations and brain circuits. In this study, we compared the efficiency of various adeno-associated viral vectors in these cell populations and demonstrated that none of the widely used promoters were, on their own, effective at driving expression of a down-stream fluorescent protein in OXT or AVP neurons. As anticipated, the OXT promoter could efficiently drive gene expression in OXT neurons and this efficiency is solely attributed to the promoter and not the viral serotype. We also report that a dual virus approach using an OXT promoter driven Cre recombinase significantly improved the efficiency of viral transduction in OXT neurons. Finally, we demonstrate the utility of the OXT promoter for conducting functional studies on OXT neurons by using an OXT specific viral system to record neural activity of OXT neurons in lactating female rats across time. We conclude that extreme caution is needed when employing non-neuron-specific viral approaches/promoters to study neural populations within the paraventricular nucleus of the hypothalamus.
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影响因子：
12.4
作者：
Cearley, CN;Wolfe, JH
通讯作者：
Wolfe, JH
影响因子：
25
作者：
Daviu, Nuria;Fuzesi, Tamas;Bains, Jaideep S.
通讯作者：
Bains, Jaideep S.
影响因子：
5.4
作者：
Grimm, Dirk;Lee, Joyce S.;Kay, Mark A.
通讯作者：
Kay, Mark A.
影响因子：
3
作者：
Gompf HS;Budygin EA;Fuller PM;Bass CE
通讯作者：
Bass CE
影响因子：
3.2
作者：
Gainer H
通讯作者：
Gainer H