Calcium silicate-based cements cause environmental stiffness and show diverse potential to induce osteogenesis in human osteoblastic cells.
Calcium silicate-based cements cause environmental stiffness and show diverse potential to induce osteogenesis in human osteoblastic cells.
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DOI：
10.1038/s41598-021-96353-0
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发表时间：
2021-08-18
影响因子：
4.6
通讯作者：
Salles LP
中科院分区：
文献类型：
--
作者：
Santiago MC;Gomes-Cornélio AL;de Oliveira LA;Tanomaru-Filho M;Salles LP
硅酸钙基水泥的显着差异在于其不透辐射剂以及硫酸钙、铝酸盐、碳酸盐和其他可能影响其生物特性的成分的存在。本研究旨在比较人成骨细胞培养物（Saos-2 细胞）中六种硅酸钙水泥的生物学特性：Bio-C Repair (Bio-C)、PBS HP (PBS-HP)、Biodentine (Biodentine)、MTA Repair HP (MTA-HP)、NeoMTA Plus (NeoMTA-P) 和 ProRoot MTA (ProRoot)。暴露于这些材料后，通过MTT、伤口愈合、细胞迁移和碱性磷酸酶活性（ALP）测定、成骨标记物（骨钙素或骨γ-羧基谷氨酸蛋白，BGLAP；碱性磷酸酶，ALPL；骨涎蛋白或分泌磷蛋白1，BNSP）的实时PCR（qPCR）分析以及茜素红染色（ARS）对细胞进行分析。奇怪的是，尽管细胞表现出理想的存活率，但接触材料 24-48 小时后迁移率较低。高级和中级细胞分化标志物 BGLAP 和 BNSP 在 Bio-C、MTA-HP 和 ProRoot 组中过表达。只有 Biodentine 组表现出 ALPL 过度表达，这是初始分化的标志。然而，除 Biodentine 外，所有组中的酶活性均较高。 NeoMTA-P、ProRoot、PBS-HP、MTA-HP 和 Bio-C 组的矿化面积显着较大。结果表明，细胞环境刚性会损害细胞流动性和成骨标记物表达的多样化模式，这是水泥暴露的结果。环境刚度表示微环境中的化学和物理刺激；例如，水泥化合物的释放有助于形成具有不同硬度的磷酸钙基质，这对于成骨细胞的迁移和分化可能是必需的或有害的。暴露于 Bio-C、PBS-HP、ProRoot、NeoMTA-P 和 MTA-HP 的细胞似乎较早进入晚期或中期分化阶段，这表明骨水泥诱导成骨的不同潜力。快速刺激成骨细胞分化的水泥可能是修复和再生目的的理想选择，因为它们会迅速导致牙本质或骨沉积。
Calcium silicate-based cements differ markedly in their radiopacifiers and the presence of calcium sulfate, aluminates, carbonates and other components that can affect their biological properties. This study aimed to compare the biological properties of six calcium silicate cements in human osteoblastic cell culture (Saos-2 cells): Bio-C Repair (Bio-C), PBS HP (PBS-HP), Biodentine (Biodentine), MTA Repair HP (MTA-HP), NeoMTA Plus (NeoMTA-P), and ProRoot MTA (ProRoot). After exposure to these materials, the cells were analyzed by MTT, wound healing, cell migration, and alkaline phosphatase activity (ALP) assays, real-time PCR (qPCR) analysis of the osteogenesis markers (osteocalcin or bone gamma-carboxyglutamate protein, BGLAP; alkaline phosphatase, ALPL; bone sialoprotein or secreted phosphoprotein 1, BNSP), and alizarin red staining (ARS). Curiously, the migration rates were low 24–48 h after exposure to the materials, despite the cells showing ideal rates of viability. The advanced and intermediate cell differentiation markers BGLAP and BNSP were overexpressed in the Bio-C, MTA-HP, and ProRoot groups. Only the Biodentine group showed ALPL overexpression, a marker of initial differentiation. However, the enzymatic activity was high in all groups except Biodentine. The mineralization area was significantly large in the NeoMTA-P, ProRoot, PBS-HP, MTA-HP, and Bio-C groups. The results showed that cellular environmental stiffness, which impairs cell mobility and diverse patterns of osteogenesis marker expression, is a consequence of cement exposure. Environmental stiffness indicates chemical and physical stimuli in the microenvironment; for instance, the release of cement compounds contributes to calcium phosphate matrix formation with diverse stiffnesses, which could be essential or detrimental for the migration and differentiation of osteoblastic cells. Cells exposed to Bio-C, PBS-HP, ProRoot, NeoMTA-P, and MTA-HP seemed to enter the advanced or intermediate differentiation phases early, which is indicative of the diverse potential of cements to induce osteogenesis. Cements that quickly stimulate osteoblast differentiation may be ideal for reparative and regenerative purposes since they promptly lead to dentin or bone deposition.
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影响因子：
4.6
作者：
Rathinam E;Govindarajan S;Rajasekharan S;Declercq H;Elewaut D;De Coster P;Martens L;Leybaert L
通讯作者：
Leybaert L
影响因子：
2.5
作者：
Maeda T;Suzuki A;Yuzawa S;Baba Y;Kimura Y;Kato Y
通讯作者：
Kato Y
影响因子：
16.6
作者：
Bangasser BL;Shamsan GA;Chan CE;Opoku KN;Tüzel E;Schlichtmann BW;Kasim JA;Fuller BJ;McCullough BR;Rosenfeld SS;Odde DJ
通讯作者：
Odde DJ
影响因子：
5
作者：
Souza, NJA;Justo, GZ;Bincoletto, C
通讯作者：
Bincoletto, C
影响因子：
2.9
作者：
Tsai, Chia-Ling;Ke, Mu-Chan;Wu, Pei-Chang
通讯作者：
Wu, Pei-Chang