Pleistocene and ecological effects on continental‐scale genetic differentiation in the bobcat (Lynx rufus)
Pleistocene and ecological effects on continental‐scale genetic differentiation in the bobcat (Lynx rufus)
复制标题
更新世和生态对山猫（Lynx rufus）大陆规模遗传分化的影响
作者：
Dawn M Reding;A. Bronikowski;W. Johnson;W. Clark
广泛分布的、移动的物种在没有明显地理障碍的情况下表现出遗传结构的可能性是一个越来越受关注的话题。然而，这些物种的结构模式和机制，特别是在广阔的空间尺度上，仍然在很大程度上未被探索。山猫遍布北美，具有许多促进基因流动的特征。为了测试是否历史，地形或生态因素影响了该物种的遗传分化，我们分析了1 kb的mtDNA序列和15个微卫星位点，从1700多个样品收集的范围。 这两种标志物类型的主要特征都涉及一个纵向渐变带，它具有一个急剧的过渡，或称缝合带，发生在大平原的沿着。因此，这些数据将美国东部的山猫与西部的山猫区分开来，没有明显的物理障碍阻碍基因流动。人口分析支持从独立的更新世避难所扩张的情景，大平原代表了一个二级接触区。两个主要谱系的子结构可能反映了创始人的影响，生态因素，人为/地形的影响或这些力量的组合。两个突出的地形特征，密西西比河和落基山脉，不支持作为显着的遗传障碍。生态区域和环境相关性解释了一个小的，但显着的遗传变异的比例。总的来说，结果表明历史过程是大规模遗传分化的主要原因，但当代力量似乎也在促进和维持结构方面发挥作用。尽管山猫的流动性和广泛的生态位，大规模的景观变化有助于显着和复杂的遗传结构模式。
The potential for widespread, mobile species to exhibit genetic structure without clear geographic barriers is a topic of growing interest. Yet the patterns and mechanisms of structure—particularly over broad spatial scales—remain largely unexplored for these species. Bobcats occur across North America and possess many characteristics expected to promote gene flow. To test whether historical, topographic or ecological factors have influenced genetic differentiation in this species, we analysed 1 kb mtDNA sequence and 15 microsatellite loci from over 1700 samples collected across its range. The primary signature in both marker types involved a longitudinal cline with a sharp transition, or suture zone, occurring along the Great Plains. Thus, the data distinguished bobcats in the eastern USA from those in the western half, with no obvious physical barrier to gene flow. Demographic analyses supported a scenario of expansion from separate Pleistocene refugia, with the Great Plains representing a zone of secondary contact. Substructure within the two main lineages likely reflected founder effects, ecological factors, anthropogenic/topographic effects or a combination of these forces. Two prominent topographic features, the Mississippi River and Rocky Mountains, were not supported as significant genetic barriers. Ecological regions and environmental correlates explained a small but significant proportion of genetic variation. Overall, results implicate historical processes as the primary cause of broad‐scale genetic differentiation, but contemporary forces seem to also play a role in promoting and maintaining structure. Despite the bobcat’s mobility and broad niche, large‐scale landscape changes have contributed to significant and complex patterns of genetic structure.
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影响因子：
3.3
作者：
Alan R. Templeton;K. Crandall;C. Sing
通讯作者：
Alan R. Templeton;K. Crandall;C. Sing
影响因子：
3.3
作者：
Yunxin Fu
通讯作者：
Yunxin Fu
影响因子：
3.3
作者：
M. Slatkin;R. Hudson
通讯作者：
M. Slatkin;R. Hudson
使用多位点基因型数据推断群体结构：连锁位点和相关等位基因频率。
影响因子：
3.3
作者：
Falush,Daniel;Stephens,Matthew;Pritchard,JonathanK
通讯作者：
Pritchard,JonathanK
对表型关联与从限制性内切核酸酶作图推断的单倍型进行分支分析。
影响因子：
3.3
作者：
Templeton,AR;Sing,CF
通讯作者：
Sing,CF