Application of Polovodova's method for the determination of physiological age and relationship between the level of parity and infectivity of Plasmodium falciparum in Anopheles gambiae s.s, south-eastern Benin.
Application of Polovodova's method for the determination of physiological age and relationship between the level of parity and infectivity of Plasmodium falciparum in Anopheles gambiae s.s, south-eastern Benin.
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DOI：
10.1186/s13071-015-0731-7
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发表时间：
2015-02-22
影响因子：
3.2
通讯作者：
Akogbéto M
中科院分区：
文献类型：
--
作者：
Anagonou R;Agossa F;Azondékon R;Agbogan M;Oké-Agbo F;Gnanguenon V;Badirou K;Agbanrin-Youssouf R;Attolou R;Padonou GG;Sovi A;Ossè R;Akogbéto M
Polovodova方法基于计算卵泡发育来估计蚊子的产卵数量。然而，一些研究人员怀疑这种方法的可靠性，因为在许多生殖营养周期后，载体中没有多次重复。正是在这种争议的背景下，我们的研究进行了评估卵泡发育的重要性，在确定产次水平在安。冈比亚公司此外，该方法的应用，使我们能够澄清媒介的恶性疟原虫感染性的演变，根据其奇偶性水平。我们使用了两种技术来确定奇偶校验的水平。冈比亚公司本研究采用了两批在实验室中产卵一定数量后繁殖的野生品系蚊虫。第一批动物使用微量移液器在卵巢中进行油注射。以同样的方式，将卵巢撕裂的经典技术（基于Polovodova方法的技术）应用于第二批。为了评估产次水平与蚊类感染性之间的关系，将Polovodova方法应用于在人身上收集的媒介。最后，使用ELISA测定单独分析这些载体的头部和胸部的恶性疟原虫抗原检测。第一批50只雌蚊“从未产过”、50只“产过一次”、50只“产过两次”和48只“产过三次”，经油法检测，未产雌蚊42只，单产雌蚊44只，双产雌蚊46只，三产雌蚊44只。总体而言，Polovodova法使用油注射技术是有效的（p > 0.05）。另一方面，在与第一批产蛋数量相似的第二批中，通过卵巢撕裂的经典技术应用Polovodova方法对经产雌性动物无效（p < 0.05）。此外，媒介感染的概率随产卵次数的增加而增加（p < 0.0001）。结果表明，Polovodova法用于冈比亚按蚊产卵量的估计是可靠的。在卵巢中使用油注射技术。该研究还表明，根据产卵的数量，病媒的传染性可能性增加。本文的在线版本（doi：10.1186/s13071-015-0731-7）包含补充材料，可供授权用户使用。
Polovodova method based on counting follicular dilatations estimates the number of egg-laying in mosquitoes. However, some researchers doubt the reliability of this method because of the absence of multiple dilatations in vectors after many gonotrophic cycles. It is in this context of controversy that our study was carried out to evaluate the importance of follicular dilatations in the determination of parity levels in An. gambiae s.s. Moreover, the application of this method allowed us to clarify the evolution of vectors’ infectivity to P. falciparum according to their parity level. We used two techniques to determine the parity level in An. gambiae s.s. We used two batches of wild strain mosquitoes reproduced after a known number of egg-laying in laboratory. The first batch was submitted to oil injection in the ovaries using a micropipette. In the same way, the classic technique of ovaries dilaceration (a technique based on the Polovodova method) was applied to the second batch. In order to assess relationship between parity level and mosquitoes’ infectivity, Polovodova method was applied on vectors collected on humans. Finally, Heads and thoraces of these vectors were individually analyzed for P. falciparum antigen detection using an ELISA assay. In the first batch including 50 female mosquitoes “never laid”, 50 “laid once”, 50 “laid twice” and 48 “three times”, oil injection technique revealed 42 nulliparous, 44 uniparous, 46 biparous and 44 triparous respectively. Overall, Polovodova method was effective using oil injection technique (p > 0.05). On the other hand, in the second batch that has a similar number of laying to the first batch, the application of Polovodova method through classical technique of ovaries dilaceration was ineffective with multiparous females (p < 0.05). Moreover, probability of vector infectivity increased with the number of egg-laying (p < 0.0001). Our results revealed that the Polovodova method is reliable for estimating the number of egg-laying in Anopheles gambiae s.s. using oil injection technique in the ovaries. The study has also showed an increased likelihood of infectivity in vectors according to the number of egg-laying. The online version of this article (doi:10.1186/s13071-015-0731-7) contains supplementary material, which is available to authorized users.
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影响因子：
1.9
作者：
HOC, TQ;CHARLWOOD, JD
通讯作者：
CHARLWOOD, JD
影响因子：
1.9
作者：
HOC, TQ;WILKES, TJ
通讯作者：
WILKES, TJ
影响因子：
1.9
作者：
CHARLWOOD, JD;LOPES, J
通讯作者：
LOPES, J
影响因子：
2.2
作者：
ELLISON, JR;HAMPTON, EN
通讯作者：
HAMPTON, EN
影响因子：
2.1
作者：
Hamer, Gabriel L.;Kitron, Uriel D.;Walker, Edward D.
通讯作者：
Walker, Edward D.