Visual detection of Hg²⁺ in aqueous solution using gold nanoparticles and thymine-rich hairpin DNA probes.
Visual detection of Hg²⁺ in aqueous solution using gold nanoparticles and thymine-rich hairpin DNA probes.
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DOI：
10.1016/j.bios.2011.05.003
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发表时间：
2011-07-15
影响因子：
12.6
通讯作者：
Liu, Guodong
中科院分区：
文献类型：
--
作者：
He, Yuqing;Zhang, Xibao;Zeng, Kang;Zhang, Sanquan;Baloda, Meenu;Gurung, Anant S.;Liu, Guodong
我们报道了一种利用金纳米颗粒（Au-NPs）和富含胸腺嘧啶（T）的发夹DNA探针对水溶液中汞离子（Hg 2+）进行视觉检测的灵敏方法。通过自组装方法将巯基化的发夹DNA探针固定在金纳米粒子表面。另一种富含胸腺嘧啶的地高辛标记的DNA探针被引入到Au-NP表面，在Hg 2+存在下，形成具有胸腺嘧啶-Hg 2 +-胸腺嘧啶（T-Hg 2 +-T）配位的DNA双链体。通过双链体上的地高辛和LFSB上的抗地高辛抗体之间的免疫反应事件，将与形成的双链体相关的Au-NP捕获在侧向流条生物组分（LFSB）的测试区上。Au-NP的积累在测试区上产生了特征性的红色条带，使得能够在没有仪器的情况下目视检测Hg 2+。在最佳实验条件下获得了0.1 nM的检测限。该方法为汞离子的检测提供了一种简单、快速、灵敏的方法，并在环境有毒汞的现场检测和使用点检测方面显示出巨大的潜力。
We report a sensitive method for visual detection of mercury ions (II) (Hg2+) in aqueous solution by using gold nanoparticles (Au-NPs) and thymine (T)-rich Hairpin DNA probes. The thiolated Hairpin DNA probe was immobilized on the Au-NP surface through a self-assembling method. Another thymine-rich, digoxin-labeled DNA probe was introduced to form DNA duplexes on the Au-NP surface with thymine-Hg2+-thymine (T-Hg2+-T) coordination in the presence of Hg2+. The Au-NPs associated with the formed duplexes were captured on the test zone of a lateral flow strip biocomponent (LFSB) by immunoreaction events between the digoxin on the duplexes and anti-digoxin antibodies on the LFSB. The accumulation of Au-NPs produced a characteristic red band on the test zone, enabling visual detection of Hg2+ without instrumentation. A detection limit of 0.1 nM was obtained under optimal experimental conditions. This method provides a simple, rapid, sensitive approach for the detection of Hg2+ and shows great promise for point-of-use and in-field detection of environmentally toxic mercury.
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