Autophagic Killing Effects against Mycobacterium tuberculosis by Alveolar Macrophages from Young and Aged Rhesus Macaques.
Autophagic Killing Effects against Mycobacterium tuberculosis by Alveolar Macrophages from Young and Aged Rhesus Macaques.
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DOI：
10.1371/journal.pone.0066985
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Purdy GE
中科院分区：
文献类型：
--
作者：
Pacheco SA;Powers KM;Engelmann F;Messaoudi I;Purdy GE
非人类灵长类动物，特别是恒河猴（Macaca mulatta， RM），为研究对结核分枝杆菌感染的免疫反应和有效控制提供了一个强大的实验模型。免疫细胞功能的改变和免疫衰老可能导致老年人对结核病的易感性增加。本研究的目的是研究年龄对幼年和老年恒河猴肺泡巨噬细胞感染后结核分枝杆菌宿主-病原体相互作用的影响。我们特别感兴趣的是，自噬巨噬细胞的分枝杆菌杀灭能力是否在老年动物中降低，因为在老年动物的其他细胞类型中观察到自噬体形成和自噬溶酶体融合减少。我们的数据表明，来自老年动物的肺泡巨噬细胞与来自年轻动物的肺泡巨噬细胞一样具有自噬清除结核分枝杆菌感染和控制分枝杆菌复制的能力。虽然我们的数据没有显示年轻和年老动物肺泡巨噬细胞对结核分枝杆菌的反应有显著差异，但这些研究首次从功能上表征了RM肺泡巨噬细胞对结核分枝杆菌的自噬清除。
Non-human primates, notably rhesus macaques (Macaca mulatta, RM), provide a robust experimental model to investigate the immune response to and effective control of Mycobacterium tuberculosis infections. Changes in the function of immune cells and immunosenescence may contribute to the increased susceptibility of the elderly to tuberculosis. The goal of this study was to examine the impact of age on M. tuberculosis host-pathogen interactions following infection of primary alveolar macrophages derived from young and aged rhesus macaques. Of specific interest to us was whether the mycobactericidal capacity of autophagic macrophages was reduced in older animals since decreased autophagosome formation and autophagolysosomal fusion has been observed in other cells types of aged animals. Our data demonstrate that alveolar macrophages from old RM are as competent as those from young animals for autophagic clearance of M. tuberculosis infection and controlling mycobacterial replication. While our data do not reveal significant differences between alveolar macrophage responses to M. tuberculosis by young and old animals, these studies are the first to functionally characterize autophagic clearance of M. tuberculosis by alveolar macrophages from RM.
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影响因子：
5.3
作者：
Boehmer, ED;Meehan, MJ;Kovacs, EJ
通讯作者：
Kovacs, EJ
影响因子：
15.3
作者：
Clemens, DL;Horwitz, MA
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作者：
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通讯作者：
Cuervo, Ana Maria
DOI：
10.1164/rccm.2201023
发表时间：
2002-07-15
影响因子：
24.7
作者：
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通讯作者：
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影响因子：
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作者：
HIGASHIMOTO, Y;FUKUCHI, Y;ORIMO, H
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