Improved detection of hepatitis B surface antigen (HBsAg) in blood donors by monoclonal radioimmunoassay-猫眼课题宝

通过单克隆放射免疫分析改进了献血者中乙型肝炎表面抗原 (HBsAg) 的检测

DOI：

发表时间：

1985

期刊：

Transfusion

影响因子：

2.9

通讯作者：

中科院分区：

医学3区

文献类型：

作者：

E. Ben‐Porath;J. Wands;S. Bar;C. Huggins;K. Isselbacher

我们评价了一种具有更高灵敏度和高特异度的单抗放射免疫分析(M-RIA)在献血员中检测乙肝表面抗原(HBs)的性能。将结果与使用多价抗体的常规放射免疫分析(P-RIA)进行比较。P-RIA对6409名没有反应的美国献血者的分析显示，M-RIA每1000名乙肝表面抗原阳性献血者中就有1.4名，或者说大约60%的乙肝表面抗原检测率提高了。此外，在P-RIA阴性的995名以色列献血者中，又发现了11名乙肝表面抗原阳性献血者。检测率提高了55%，与美国献血者的观察结果相似。由于几名新确认的HBs Ag阳性献血者的核心抗原(抗-HBc)抗体是最近或过去接触过乙肝的唯一血清学证据，我们用M-RIA对另外68名抗-HBc阳性的人进行了研究。发现26%(18/68)的患者仅对M-RIA有反应，而对P-RIA无反应。提示存在P-RIA检测不出HBs Ag的献血者。

We evaluated the performance of a monoclonal radioimmunoassay (M‐RIA) with enhanced sensitivity and high specificity for hepatitis B surface antigen (HBsAg) in blood donors. The results were compared to a conventional RIA that used polyvalent antibodies (P‐RIA). Analysis of 6409 American blood donors not reactive by P‐RIA revealed an additional 1.4 HBsAg‐positive donors per 1000 by M‐RIA, or an approximate 60 percent, improvement in HBsAg detection rate. Furthermore, in 995 Israeli blood donors negative by P‐RIA, 11 additional HbsAg‐positive donors were identified. The 55 percent improvement in detection rate was similar to that observed with American blood donors. Since several of the newly identified HBsAg‐positive blood donors had antibodies to the core antigen (anti‐HBc) as the only serologic evidence of recent or past hepatitis B exposure, we studied an additional 68 anti‐HBc‐ positive individuals with the M‐RIA. It was found that 26 percent (18/68) reacted only by M‐RIA and not by P‐RIA. These findings suggest that there are blood donors with HBsAg undetectable by P‐RIA.

Monoclonal IgM radioimmunoassay for hepatitis B surface antigen: high binding activity in serum that is unreactive with conventional antibodies.